Antibacterial Activity and Mechanism of Gallic acid on Staphylococcus aureusChinese Full Text

LU Xiao,ZHOU Lei,XIE Kun-peng,JING Yi-ren,YUN Bao-yi,WANG Ye-ju,XIE Ming-jie(School of science,Liaoning Normal University,Key Laboratory of Biotechnology and Drug of Liaoning Province,116081,Dallian,China)

Abstract: To investigate the Antibacterial Activity and Mechanism of Gallic acid on Staphylococcus aureus.The antibacterial activity of Gallic acid was carried out by utilizing oxford cup and 2,3,5-triphenyl tetrazolium chloride（TTC）,and the mechanisms of Gallic acid on Staphylococcus aureus were systematically investigated using determining membrane penetrability,the change of SDS-PAGE protein spectra and so on.It is found that Gallic acid has antibacterial activity on Staphylococcus aureus,and the minimum inhibitory concentrations（MIC）of Gallic acid is 0.01 mg·mL-1.The results of the effects of Gallic acid on cell membrane permeability of Staphylococcus aureus has a significant influence to the cytoplasm extravasation;When it drugs 5h,the rate of change about conductivity was up to 6.38% treated with Gallic acid,while macromolecules rate of change of absorbance has reached the maximum,compared with the control group increased by 270%.It could destroy the integrality of cell membrane.According the soluble protein of Staphylococcus aureus by Gallic acid,when it dugs 20h,the quartity of protein don’t change greatly.Gallic acid showed obvious antibacterial activity to Staphylococcus aureus.The mechanism is that It could destroy the integrality of cell membrane.

Keywords：

Gallic acid; Staphylococcus aureus; Antibacterial activity; Antibacterial mechanism;

DOI：
10.13629/j.cnki.53-1054.2012.04.004
Series：
Subject：
Classification Code：
R285

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Antibacterial Activity and Mechanism of Gallic acid on Staphylococcus aureusChinese Full Text

LU Xiao,ZHOU Lei,XIE Kun-peng,JING Yi-ren,YUN Bao-yi,WANG Ye-ju,XIE Ming-jie(School of science,Liaoning Normal University,Key Laboratory of Biotechnology and Drug of Liaoning Province,116081,Dallian,China)

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