Chinese Journal of Clinical Laboratory Science. 2014, (05) Core

Different inhibitory effects of methotrexate enantiomers on acute lymphoid leukemia cell linesChinese Full Text

ZHANG Pei;SUO Mei-fang;CHEN Yang;HE Xiao-dong;SHEN Zuo-jun;Center for Clinical Laboratory,the Affiliated Provincial Hospital of Anhui Medical University;

Abstract: Objective To investigate the different inhibitory effects of methotrexate（ MTX） enantiomers [including L-（ +） MTX,D-（-） MTX,（ ±） MTX（ D∶ L =1∶ 9 ~9∶ 1） ]on the proliferation of lymphoid leukemia cell lines BALL-1 and CCRF-CEM. Methods BALL-1 and CCRF-CEM cells were affected by the monomer or mixed type of MTX enantiomer. The inhibitory rates of proliferation of the cells were determined by MTT method. The morphology of the cells was inspected by fluorescence microscope. The phases of cell cycle were assayed by propidium iodide staining and flow cytometry. The apoptosis levels were detected by electrophoresis loading DNA ladder. Results Compared with the control groups,MTX enantiomers inhibited the growth of BALL-1 and CCRF-CEM cells in timeand dose-dependent manner. The inhibitory efficacy of（ ±） MTX（ D∶ L = 1∶ 9 and 2∶ 8） was higher than those of both L-（ +） MTXand D-（-） MTX,and the inhibitory efficacy of L-（ +） MTX was higher than those of（ ±） MTX（ D∶ L = 3∶ 7,4∶ 6,5∶ 5,6∶ 4,7∶ 3, 8∶ 2 and 9∶ 1） and D-（-） MTX,The inhibitory efficacy of D-（-） MTX was the lowest. The cell cycles were arrested in S phase,and the apoptosis bands were found in DNA ladder electrophoresis. There were significant differences of the cell cycle among parental BALL-1,L-（ +） MTX / BALL-1and D-（-） MTX / BALL-1（ P ＜ 0. 01）,or parental CCRF-CEM,L-（ +） MTX / CCRF-CEM and D-（-） MTX/CCRF-CEM（ P ＜0. 01）. Conclusion The inhibitory effects of MTX enantiomers on proliferation of BALL-1 and CCRFCEM cells presented chiral-selective in chemical structure.

Keywords：

methotrexate; enantiomers; BALL-1 cell; CCRF-CEM cell;

DOI：
10.13602/j.cnki.jcls.2014.05.16
Series：
Subject：
Classification Code：
R733.7

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Different inhibitory effects of methotrexate enantiomers on acute lymphoid leukemia cell linesChinese Full Text

ZHANG Pei;SUO Mei-fang;CHEN Yang;HE Xiao-dong;SHEN Zuo-jun;Center for Clinical Laboratory,the Affiliated Provincial Hospital of Anhui Medical University;

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