Progress in Construction Methods of Diverse Mutated Gene Libraries for ProteinChinese Full Text
ZHANG Xiu-yan~1 HE Guo-qing~2(1 Department of Food Chemistry and Molecular Biology, Huazhong Agriculture University, Wuhan 430070, China)(2 Department of Food Science and Nutrition, Zhejiang University Hangzhou 310029, China)
Abstract: Directed evolution has been proven to be an effective method for proteins engineering. In the past few years, many new protocols for directed evolution were developed on the basis of oligonucleotide -directed random mutation, error-prone PCR and DNA shuffling. These new methods and their characteristics were summarized to provide a reference for choosing an approach to modify specific protein properties. The recent researches indicated that the combined directed evolution, coupling the directed evolution with rational design and computational design approaches respectively, is becoming increasingly important in protein engineering and is becoming the new direction for directed evolution.
- DOI：
10.13523/j.cb.20061011
- Series：
- Subject：
- Classification Code：
Q78
- Mobile Reading
Read on your phone instantly
Step 1Scan QR Codes
"Mobile CNKI-CNKI Express" App
Step 2Open“CNKI Express”
and click the scan icon in the upper left corner of the homepage.
Step 3
Scan QR Codes
Read this article on your phone.
- CAJ Download
- PDF Download
Download the mobile appuse the app to scan this coderead the article.
Tips: Please download CAJViewer to view CAJ format full text.
Download: 665 Page: 52-58 Pagecount: 7 Size: 204K
Citation Network
Related Literature
- Similar Article
- Reader Recommendation
- Associated Author