Apoptosis of K562 cells induced by a new immunosuppressive agent FTY720 and its mechanismChinese Full Text
ZHANG Chen-hao;LI Yan;CHEN Wei;CHEN Shuang;FANG Fang;ZHAO Liang-zhong;Department of Pathogeny and Microbiology,Department of Medical Laboratory,Jilin Medical College;
Abstract: Objective To study the apoptosis of K562 cells induced by a new immunosuppressive agent FTY720 and its mechanism,and to provide experimental basis for the treatment of leukemia in clinic.Methods The K562 cells were cultured in vitro and divided into blank control group and FTY720 treatment group.The K562 cells in FTY720 treatment group were treated with 6μmol·L-1 FTY720 for 3,6,and 12 h,or treated with different concentrations of FTY720（2,4,6,8μmol·L-1）for 24 h.The apoptosis,level of reactive oxygen species（ROS）,mitochondrial membrane potential（MMP）and cell cycle were measured by flow cytometry.The inhibitory rate of proliferation of K562 cells after treated with FTY720 was detected by WST-1reducting assay.Results The results of flow cytometry showed that the percentages of apoptotic cells were increased after treated with6μmol·L-1 FTY720 for 3,6,and 12 hwith the prolongation of time compared with blank control group（P＜0.01）.The percentages of apoptotic cells were also increased after treated with different concentrations of FTY720 for 24hcompared with blank control group（P＜0.01）.Compared with blank control group,the ROS levels were increased with the increasing of FTY720 concentration,while the MMP was decreased（P＜0.01）.Compared with blank control group,the percentage of cells at G0/G1 phase was increased,while those at S and G2/M phases were decreased with the increasing of FTY720concentration（P＜0.05）.The WST-1reduction assay results indicated that the inhibitory rates of proliferation of K562 cells after treated with 2,4,6,and 8μmol·L-1 FTY720 for 72h were（24.0±4.1）%,（46.4±3.9）%,（67.0±4.8）%,and（88.2±5.6）%,respectively,compared with blank control group.The concentration of FTY720 to result the inhibitory rate of 50%（IC50）on K562 cells was5.5μmol·L-1.Conclusion FTY720 could inhibit the proliferation of K562 cells by blocking cell cycle and inducing apoptosis through provoking ROS.
- DOI：
10.13481/j.1671-587x.20140607
- Series：
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- Classification Code：
R965
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