Ultraviolet mutagenesis and resistance selection of daptomycin-producing strain（ATCC31568）Chinese Full Text
Liu Ti-yan;Xing Yu-hua;Dai Su-qin;Zhang Bu-chang;Liu Gang;Chen Hui-peng;School of Life Sciences,Anhui University;Institute of Bioengineering,Academy of Millitary Medical Sciences;College of Life Science,Jilin University;
Abstract: Objective To set up an efficient method for increasing the production of daptomycin. Methods Ultraviolet mutagenesis and resistance selection was used to increase the production of daptomycin from original strain of Streptomyces roseosporus ATCC31568. Results The results showed that the dose of UV irradiation was determined 45s in term of lethality rate and positive mutation, the minimum inhibitory concentration（MIC） of the Rifampicin and Gentamicin were 1.4 μg/mL＆1.0 μg/mL, the final broth in flasks was determined by HPLC. High daptomycin-producing mutant Ge-27 was isolated in the screening experiments, and its yield of daptomycin reached 79.44 mg/L,which was 42.2% higher than its parental strain. Conclusion The mutants was obtained whose production of daptomycin could be increased by UV mutagenesis and resistance selection.
- DOI：
10.13461/j.cnki.cja.005251
- Series：
- Subject：
- Classification Code：
Q933
- Mobile Reading
Read on your phone instantly
Step 1Scan QR Codes
"Mobile CNKI-CNKI Express" App
Step 2Open“CNKI Express”
and click the scan icon in the upper left corner of the homepage.
Step 3
Scan QR Codes
Read this article on your phone.
- HTML
- CAJ Download
- PDF Download
Download the mobile appuse the app to scan this coderead the article.
Tips: Please download CAJViewer to view CAJ format full text.
Download: 475 Page: 664-669 Pagecount: 6 Size: 507K
Citation Network
Related Literature
- Similar Article
- Reader Recommendation
- Associated Author