Effect of dapA gene deletion on L-threonine production by Escherichia coliChinese Full Text
ZHANG Li,LI Wei-xing,YANG Xiao-zhi,ZHANG Jun-sheng(Jiangsu Profession and Technical College of Animal Science and Veterinary Medicine,Taizhou 225300,China)
Abstract: Using Red recombination technique,the dapA gene in Escherichia coli ’K12’was replaced,then a mutant without dapA gene was gained,and it was named as K12△dapA.Thus the K12△dapA and Escherichia coli ’K12’were both fermentated under same condition for 48 h,the L-threonine content in K12△dapA broth was 3.71 g/L,which was 3 times of that in Escherichia coli ’K12’broth.The results indicated that dapA gene deletion improved the accumulation of L-threonine.
- DOI：
10.13432/j.cnki.jgsau.2011.04.005
- Series：
- Subject：
- Classification Code：
TQ922.9
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