Analysis on the Internal Transcribed Spacers（ ITS） Sequences and Phylogenetics of Chieh-qua and its Affinis Cucurbitaceae Germplasm ResourcesChinese Full Text
ZHAO Qin;XIE Da-sen;HE Xiao-ming;CHEN Ren-fang;PENG Qing-wu;LUO Shao-bo;Vegetable Research Institute,Guangdong Academy of Agricultural Sciences;Key Sericultural Laboratory of Ministry of Agriculture /College of Biotechnology,Southwest University;
Abstract: 56 chieh-qua and 7 other cucurbits germplasm resources were collected from south China,subsequently rDNA-ITS amplication and sequencing were performed. Combining ITS sequences of several cucurbitaceous plants downloaded from GenBank,the lengths,variable sites,G + C content,genetic divergences,homologies,phylogenetics,and system position were analyzed by bioinformatic softwares. ITS complete sequences of chieh-qua and its affinis cucurbitaceae germplasm resources were obtained. The full-length of chieh-qua ITS was612- 617 bp,G + C content were 59. 97%- 61. 07%. The sequence alignment results showed that there were 94 variable sites（ ITS1: 47,ITS2: 40,5. 8S: 7） among chieh-qua germplasm resources,some of which had significant species-specific characteristics and could be used as DNA fingerprint marker. 56 chieh-qua germplasm materials were clustered into 3 groups based on ITS sequences variance,12-2-3 was ranked into the first branch,’Heimaojiegua H2251’ and ’ H5FA’ were classified into the second and third branch respectively,and the rest materials were grouped into the fourth and fifth group. Compared with the other cultivated species,the genetic divergence of chieh-qua material 12-2-3 was the biggest ranging from 3. 3 to 4. 6,and there are large differences on agronomic traits and resistance between 12-2-3 and others,leading to be used as crossbreeding parent. The system position of chieh-qua was between Indomelothria blumei（ GU799496） and Dactyliandra welwitschii（ HQ201973）,and had the closest relationship with Indomelothria blumei. Mrbayes software analysis showed that Zehneria thwaitesii（ AM981145）,Ctenolepis cerasiformis（ AM981142）,Cucumis melo（ AM36377）,Dactyliandra welwitschii（ HQ20 1973）,Trochomeria macrocarpa（ AM981141） were the most primordial species,the system evolution order was Cucumis melo（ AM36377） → Cucurbita moschata,Trichosanthes yunnanensis,Momordica charantia,Luffa aegyptiaca,Citrullus lanatus（ FJ915098）,Lagenaria siceraria → Benincasa hispida,Benincasa hispida var. chieh-qua→ Cucumis sativus. The phylogeny of chieh-qua and its affinis cucurbitaceae germplasm resources was investigated by using ITS sequences and it would provide useful information for their breeding,germplasm DNA fingerprint identification,classification and evolution and comparative analysis,etc.
Keywords：
chieh-qua; cucurbitaceous crops; germplasm resources; rDNA-ITS; sequence analysis; phyletic evolution;
- DOI：
10.13430/j.cnki.jpgr.2014.05.026
- Series：
- Subject：
- Classification Code：
Q943.2
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