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Identification of Egg-shaped Ruantiao Baisha Loquat Mutant GermplasmChinese Full Text

CHEN Fang-yong;XIE Li-xue;NI Hai-zhi;WANG Yin;HONG Ting;REN Zheng-chu;Citrus Institute of Zhejiang Province;Fruit Research Institute,Fujian Academy of Agricultural Sciences;Forest and Special Plant Station of Luqiao District,Taizhou City in Zhejiang Province;

Abstract: Egg-shaped ruantiao baisha mutant and 30 loquat germplasms from China,Japan and New Zealand were used as plant material and contrast,respectively in this paper. Identification of the mutant germplasm were constructed with SRAP molecular marker based on the morphological investigation. 18 pairs of primers were selected from 96 pairs of SRAP primers. Finally,135 loci were detected,of which 102 were polymorphic and the polymorphic percentage was 75. 6%. The genetic similarity ranged from 0. 53 0. 89. 31 white pulp loquat germplasms were divided into four groups at the similar coefficient of 0. 65. The loquat germplasms came from Zhejiang province were clustered together into the first group. The second group contained 19 germplasms which came from China,Japan,and New Zealand. The third group included Shanghai baisha and Guangxin bami. The fourth group included Bailizhi and Longcai bai. The genetic simlity between egg-shaped ruantiao baisha and the other contrasted germplasms ranged from 0. 55 to 0. 88. It had the maximum GS with Bailizhi( GS = 0. 88),and minimun GS with Wugongbai( GS= 0. 55),while the GS between egg-shaped ruantiao baisha and ruantiao baisha was only 0. 73. Study of biological characters showed that egg-shaped ruantiao baisha and ruantiao baisha had significant differences among fruit shape,seed number,and edible rate. As the comprehensive results,the egg-shaped ruantiao baisha had significant genetic variation with ruantiao baisha loquat,and it should be a mutant germplasm. The study would be of great practical significance to new varieties innovation and protection of intellectual property rights for loquat mutantgermplasm.
  • DOI:

    10.13430/j.cnki.jpgr.2014.05.010

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  • Classification Code:

    S667.3

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