Down-regulation of TSPO expression doesn’t affect the productions of TNF-α,IL-1β and IL-6 in LPS-stimulated BV-2 microgliaChinese Full Text
DOU Xiaofeng;ZHANG Yafei;CHEN Qiang;LI Wenjiao;LI Yang;YU Chundong;SU Xinhui;Department of Nuclear Medicine,First Affiliated Hospital,Xiamen University;State Key Laboratory of Cellular Stress Biology,School of Life Sciences,Xiamen University;
Abstract: Objective To evaluate the effect of translocator protein（ TSPO,Mr18 000） on the productions of TNF-α,IL-1βand IL-6 in lipopolysaccharide（ LPS）-stimulated BV-2 cells. Methods RNA interference technique was used to decrease TSPO expression in BV-2 cells. Western blotting was performed to assess the level of TSPO protein in BV-2 cells transfected with TSPO siRNA. Then the mRNA and protein levels of TNF-α,IL-1β,and IL-6 were measured in LPS-stimulated BV-2 cells by real-time quantitative PCR（ qRT-PCR） and ELISA,respectively. Results The level of TSPO protein obviously decreased in BV-2 cells transfected with TSPO siRNA. However,knockdown of TSPO had no effect on the productions of TNF-α,IL-1βand IL-6 in LPS-stimulated BV-2 cells. Conclusion Down-regulated TSPO is not directly involved in regulating TNF-α,IL-1βand IL-6 productions induced by LPS in microglia.
- DOI：
10.13423/j.cnki.cjcmi.007080
- Series：
- Subject：
- Classification Code：
R741
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