siRNA-mediated inhibition of ErbB2 expression and its effect on breast cancer cell growthChinese Full Text
JIANG Kai,YANG Zhi-hong,WANG Zhao-yun,XU Xiao-jie,CHENG Long,ZHANG Hao,HAN Yong-jian,YE Qi-nong Beijing Institute of Biotechnology,Beijing 100850,China
Abstract: AIM: To construct a vector of ErbB2 small interfering RNA（siRNA）,and to investigate its effect on ErbB2 expression and the growth of ZR75-1 breast cancer cells.METHODS: Two ErbB2-siRNAs were designed and inserted into the pSliencer 2.1-U6 neo vector.After confirmed by restriction and DNA sequencing,siRNA vectors were co-transfected with the FLAG-ErbB2 expression vector into human embryonic kidney 293T cells,or transfected into SKBR3 and ZR75-1 breast cancer cells.The effects of siRNAs on the expression of ErbB2 were identified by Western blot and the proliferation of ZR75-1 cells was repressed.RESULTS: Two siRNAs could effectively inhibit the expression of exogenous and endogenous ErbB2 proteins,and could repress the growth of ZR75-1 cells.CONCLUSION: ErbB2 siRNAs effectively inhibit the expression of ErbB2,thus repressing the growth of ZR75-1 cells.
- DOI：
10.13423/j.cnki.cjcmi.005912
- Series：
- Subject：
- Classification Code：
R737.9
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