Optimization of Flash Extraction Process of Active Ingredients from Eucommiae FoliumChinese Full Text
CHEN Hai-li;ZHANG Feng;XU Jing;ZHANG Jing-jing;XU Lan-bo;LI Qin;Pharmaceutical College of Henan University;Engineering Laboratory of Henan Province for Eucommia ulmoides Cultivation and Utilization;Institute of Chinese Medicine of Henan University;
Abstract: Objective: To optimize flash extraction process of active components from Eucommiae Folium. Method: HPLC was employed to determine contents of chlorogenic acid and geniposidic acid with mobile phase of methanol-water-glacial acetic acid（ 15∶ 85∶ 1）,flow rate of 1. 0 mL·min-1and detection wavelength at237 nm. Taking yields of chlorogenic acid and geniposidic acid as index,based on single factor tests,orthogonal test was adopted to optimize extraction process with ethanol concentration,liquid-solid ratio,extracting time and times as factors. Result: Optimal process conditions were as follows: extracted thrice with 25 times the amount of70% ethanol for 6 min each time; yields of chlorogenic acid and geniposidic acid were 1. 82% and 0. 47%,respectively. Conclusion: This optimized process is simple,accurate and suitable for extracting active ingredients from Eucommiae Folium.
- DOI：
10.13422/j.cnki.syfjx.2014190019
- Series：
- Subject：
- Classification Code：
R284.2;R283.6
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