Emodin relaxes rat cerebral basilar artery via activation of large-conductance Ca2+-activated K+channels in vascular smooth muscle cellsChinese Full Text
Zhang Chuanlin;Ma Ketao;Li Li;Si Junqiang;School of Medicine,Shihezi University;The Key Laboratory of Xinjiang Endemic and Ethnic Diseases;
Abstract: Objective Aim: To study the effect of emodin on BK Ca channels in cerebral basilar artery smooth muscle cells.Method: The whole-cell patch clamp recording technique was used to investigate the regulatory mechanism of emodin on smooth muscle cells.Results: Emodin enhanced the outward current in SD rat cerebral basilar arteries smooth muscle cells;10-5mol / L emodin enhanced the outward current（+ 60mV） of BASMCs 1.33 ± 0.08 times than control（n = 6,P ＜ 0.05）.TEA,a selective BK Ca channel blocker,significantly inhibited the emodin-induced the outward current;Single smooth muscle cells were incubated in extracellular fluid with no Ca2 +30 minutes,the enhancement of emodin on the membrane current of BASMCs disappeared.Nimodipine,a selective L-Ca2 +channel blocker,significantly inhibited the emodin-induced BK Ca channels current.BAPTA-AM was added in the pipette solution,and inhibited the emodin-induced BK Ca channels current.Conclusion: Emodin relax cerebral basilar artery smooth muscle via activation of BKCa channel.
Keywords：
emodin（大黄素）; cerebral basilar artery; smooth muscle cell; BK Ca channels; Ca2+; whole-cell patch clamp recording;
- DOI：
10.13412/j.cnki.zyyl.2013.04.053
- Series：
- Subject：
- Classification Code：
R285
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