Antitumor activity and structure-activity relationship of four steroids from Fomitiporia ellipsoideaChinese Full Text

SONG Ming-Jie;BAO Hai-Ying;BAU Tolgor;LI Yu;Engineering Research Centre of Edible and Medicinal Fungi, Ministry of Education, Jilin Agricultural University;College of Medicinal Materials, Jilin Agricultural University;

Abstract: Four steroids, ergosterol（ergosta-5,7,22-triene-3β-ol）, ergosterol peroxide（5α,8α-epidioxyergosta-6,22-dien- 3β-ol）, ergosta-7,22-dien-3β-yl palmitate and ergosta-4,6,8（14）,22（23）-tetraen-3-one, were separated from petroleum ether extract of Fomitiporia ellipsoidea fruiting body by silica gel column chromatography and recrystallization. The structure was identified byNMR and MS, and compared with standardized product. The cytotoxicity of four steroids to Hep G2, MCF-7, Hela and A549 cell lines were assessed with trypan blue method. In vivo tumor growth inhibition was assayed with H22 tumor mouse model. The cytotoxicity test results showed that ergosterol and ergosterol peroxide had better inhibition effects to Hep G2, MCF-7, Hela and A549 cell lines, especially ergosterol whose inhibition rates to the four cell lines were 82.89%, 74.33%, 50.03% and 69.33% under the dose of 50·g/m L, and the IC₅₀ values were 20.61·g/m L, 37.18·g/m L, 49.89·g/m L and 38.74·g/m L respectively. The inhibitory activity of ergosterol on Hep G2 was stronger than that on other tumor cell lines. The test results in vivo showed that the inhibition rates of ergosterol and ergosta-4,6,8（14）,22（23）-tetraen-3-one against Hep G2 were 60.75% and 63.21% respectively under the dose of 50mg/m L. The spleen index in H22 tumor mouse intragastric administration by ergosterol was increased significantly. The analysis showed that the structure-activity relationship of steroids against tumor activity was obvious after replacing with hydroxyl or carbonyl at C-3.

Keywords：

Fomitiporia ellipsoidea; steroids; antitumor activity; structure-activity relationship;

DOI：
10.13346/j.mycosystema.140032
Series：
Subject：
Classification Code：
R284;R285

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Antitumor activity and structure-activity relationship of four steroids from Fomitiporia ellipsoideaChinese Full Text

SONG Ming-Jie;BAO Hai-Ying;BAU Tolgor;LI Yu;Engineering Research Centre of Edible and Medicinal Fungi, Ministry of Education, Jilin Agricultural University;College of Medicinal Materials, Jilin Agricultural University;

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