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Chinese Journal of Biotechnology. 2014, (03) Core

Preparation and immunogenicity of silk fibroin/chitosan microspheres for DNA vaccine delivery against infectious bursal disease virusChinese Full Text

Yan Liu;Zhiqiang Lv;Cun Zhang;Xingrong Zhu;Tuanyuan Shi;Shi Zhong;Zhiqi Meng;State Key Laboratory Breeding Base for Zhejiang Sustainable Plant Pest and Disease Control,the Sericultural Research Institute,Zhejiang Academy of Agricultural Science;Institute of Animal Husbandry and Veterinary Medicine,Zhejiang Academy of Agricultural Sciences;

Abstract: To evaluate the immunities of biodegradable microsphere as a release delivery system for DNA vaccine against Infectious Bursal Disease Virus,in our study,silk fibroin/chitosan microsphere adjuvant was prepared with a precipitation/coacervation method.Both glutaraldehyde and Na2SO4 solution were used in cross-linking.No immune chicken were intramuscularly inoculated at 14 day-old and boosted 2 weeks later.The results show that glutaraldehyde destroyed the DNA activity of the vaccine whereas Na2SO4 solution did not.Factors of the chitosan concentration 0.5%（pH 5.0）,silk fibroin concentration 0.6%,plasmid DNA（500 μg/mL） dissolved in 2% Na2SO4 solution were optimized to produce microsphere,with a loading capacity of 89.14%.The average particle size of SF-CS/pCI-VP2/4/3 microsphere is 1.98 μm,and it can protect the loading DNA vaccine from DNaseⅠdigestion.Data from anti IBDV ELISA antibodies in the serum show that immunization activity of the microsphere groups were generally higher than plasmid vaccine group（P＜0.05）,and the SF/CS compound microspheres group was better than that of sole CS microsphere group.The developed SF/CS microspheres are a very promising vaccine delivery system.
Keywords：

microsphere vaccine; silk fibroin; chitosan; IBDV; VP2/4/3; immunogenicity;

  • DOI：

    10.13345/j.cjb.130344

  • Series：

  • Subject：

  • Classification Code：

    S855.3

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