EFFECT OF SODIUM BUTYRATE ON APOPTOSIS AND CALCIUM CONCENTRATION IN HUMAN COLORECTAL CANCER CELLS HT-29Chinese Full Text

SUN Su-xia,LI Wen-jun1,CHEN Si-qiang2,LUO Hai-ji,ZOU Fei1(Department of Nutrition and Food Hygiene,1Department of Occupational Health and Occupational Medicine,School of Public Health and Tropical Medicine,Southern Medical University,Guangzhou 510515;2Fo Shan Entry-Exit Inspection and Quarantine,Foshan 528000,China)

Abstract: Objective To investigate the mechanism of sodium butyrate（NaB） on apoptosis in human colorectal cancer cell line HT-29.Method The apoptosis of HT-29 induced by NaB was confirmed by hoechst33342 staining and AnnexinV+ PI assay.The concentration of intracellular and endoplasmic reticulum（ER） Ca2+was detected by an inverted laser scanning confocal microscope.Results NaB induced the apoptosis of HT-29 cells in time-and dose-dependence.The pro-apoptotic effect of NaB was further corroborated with the measurement of cleavage of poly ADP-ribose polymerase（PARP）,A marked increase in the cleavage of PARP was noted after treatment with 2.5 mmol/L NaB for 24 h and Ca2+ was released from ER,which in turn caused extracellular Ca2+ influx in HT-29 cells.Conclusion The apoptosis induced by NaB in human colorectal cancer cells is correlated with the concentration of intracellular and ER Ca2+.

Keywords：

sodium butyrate; human colorectal cancer cell; apoptosis; Ca2+;

DOI：
10.13325/j.cnki.acta.nutr.sin.2013.01.028
Series：
Subject：
Classification Code：
R735.34

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EFFECT OF SODIUM BUTYRATE ON APOPTOSIS AND CALCIUM CONCENTRATION IN HUMAN COLORECTAL CANCER CELLS HT-29Chinese Full Text

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