CHANGES OF SERUM LEPTIN SERUM INSULIN AND SUPPRESSORS OF CYTOKINE SIGNALING-3 GENE EXPRESSION IN HYPOTHALAMUS OF OBESE RATS FED HIGH-FAT DIETChinese Full Text

ZHANG Xu,LIU Zheng-juan1,ZHAI Na2(The Second Hospital of Jiaxing,Jiaxing 314000;1The Second Hospital Affiliated to Dalian Medical University,,Dalian 116027;2The First People’s Hospital of Shenyang,Shenyang 110041,China)

Abstract: Objective To observe the concentration of serum leptin,serum insulin and changes of suppressorsof-cytokine-signaling 3（SOCS-3） gene expression in hypothalamus of obese rats fed with high-fat diet,and to explore the pathogenesis of leptin resistance and insulin resistance.Method Four-week-old healthy male SD rats were fed with high-fat diet to set up the animal model of dietary obesity.The concentration of serum leptin,serum insulin and C peptide was measured with radioimmunoassay.The concentration of glucose was measured with glucose oxidase method.Expression of SOCS-3 mRNA in the hypothalamus was detected with semiquantitative RT-PCR.Results（1） The body weight of rats fed high-fat diet was increased significantly than that in control group;（2） The concentration of serum leptin,insulin,glucose and C peptide in rats of obese group was elevated obviously compared with control group;（3） The expression of SOCS-3mRNA in obese group was increased markedly compared with control group;（4） There were significant positive relationship between SOCS-3 mRNA expression in hypothalamus and serum leptin,insulin concentration in obese group.Conclusion High-fat diet can lead to leptin resistance,insulin resistance and upregulation of SOCS-3 expression,which may act as the negative factors of JAK/STAT pathway that result in leptin resistance and insulin resistance in obese rats.

Keywords：

obesity; suppressors of cytokine signaling; insulin resistance; leptin resistance;

DOI：
10.13325/j.cnki.acta.nutr.sin.2010.04.029
Series：
Subject：
Classification Code：
R589.2

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CHANGES OF SERUM LEPTIN SERUM INSULIN AND SUPPRESSORS OF CYTOKINE SIGNALING-3 GENE EXPRESSION IN HYPOTHALAMUS OF OBESE RATS FED HIGH-FAT DIETChinese Full Text

ZHANG Xu,LIU Zheng-juan1,ZHAI Na2(The Second Hospital of Jiaxing,Jiaxing 314000;1The Second Hospital Affiliated to Dalian Medical University,,Dalian 116027;2The First People’s Hospital of Shenyang,Shenyang 110041,China)

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