Expression of mTOR in breast cancer and the effect of mTOR inhibitor on the cell proloferation and apoptosis of breast cancer cellsChinese Full Text
LI Yuan-yuan;TIAN Hong-yan;LIU Yu-qing;Department of Morphology,Weifang Medical University;Department of Pathology,Weifang Medical University;
Abstract: Purpose To investigate the expression of mTOR in breast cancer,and to observe the effect of CCI-779 on proliferation and apoptosis of MDA-MB-231 cell. Methods Immunohistochemical staining was used to detect the expression of mTOR protein in breast cancer tissue and MDA-MB-231 cell. MTT method was used to show effect of CCI-779 on proliferation of MDA-MB-231 cell. AnnexinV-FITC /PI method was used to show effect of CCI-779 on apoptosis of MDA-MB-231 cell. Results 54. 9% in 71 cases of breast cancer tissue could express mTOR protein,the expression was significantly higher than in 32 cases of normal tissue（ 21. 9%）,mTOR protein was also detected in MDA-MB-231 cell,CCI-779 could inhibit the proliferation of MDA-MB-231 cell,and show a dose-and time-dependent,but CCI-779 could not induce apoptosis of MDA-MB-231 with AnnexinV-FITC /PI assay. Conclusion mTOR is closely related to the formation of breast cancer,CCI-779 has strong activity against MDA-MB-231 cell,it has prospect for treatment of breast cancer in the future.
Keywords：
- DOI：
10.13315/j.cnki.cjcep.2014.09.006
- Series：
- Subject：
- Classification Code：
R737.9
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