Research on the correlation of TLR4,zinc finger protein A20 in peripheral blood monocyte and tissue factor in acute coronary syndrome patientsChinese Full Text
YU Chun-yang;WANG Zhao-jun;PU Xiao-jun;CHEN Xiao-jie;Department of Cardiology,the Affiliated Hospital of Jiangsu University;
Abstract: Objective: To investigate the relation between the expression levels of toll-like 4/A20 mRNA in peripheral blood mononuclear cells（ PBMC） and tissue factor,tissue factor passway inhibitor（ TFPI） in plasma of patients with acute coronary syndrome（ ACS）. Methods: The patients of coronary artery diseases（ CAD） were divided into the group of ACS（ n =31）,there were 15 patients with acute myocardial infarction（ AMI）,and 16 patients with unstable angina pectoris（ UAP）; and stable angina pectoris（ SAP,n = 21） group; the group of control were a total of 20 cases. The expressions of TLR4 and A20 mRNA were detected by real-time fluorescence quantitation polymerase chain reaction（ RT-PCR）. The concentrations of plasma tissue factor and TFPI were measured by enzyme-linked immunosorbent assay（ ELISA）. Results: There was a significant correlation between TLR4 mRNA and A20 mRNA in peripheral blood mononuclear cells（ r = 0. 640,P = 0. 002）. The expressions of A20 and TLR4 mRNA in monocytes in patients with ACS were higher than patients with SAP and healthy persons（ P ＜ 0. 001）. Comparing with the single lesion group,TLR4 and A20 mRNA positive expression in the multivessel branch lesion group were increased（ P =0. 006,P =0. 000）. The levels of both tissue factor and TFPI in patients with ACS were significantly higher than the group of SAP and controls（ P ＜ 0. 01）. Plasma tissue factor was statistically significant between the multivessel disease group and the single lesion group（ P = 0. 017）. The expression of TLR4 in PBMC group was positively related with the level of TF in ACS（ r = 0. 718,P = 0. 000）. Conclusion: The increased expression of TLR4 in monocytes was related to atherosclerotic plaque instability as well as coronary artery lesion degree. TLR4-mediated inflammatory pathway activation may promote clotting abnormalities and coronary artery thrombosis in patients with ACS.
Keywords：
acute coronary syndrome; toll-like receptor 4; zinc finger protein A20; tissue factor; tissue factor passway inhibitor;
- DOI：
10.13312/j.issn.1671-7783.y130036
- Series：
- Subject：
- Classification Code：
R541.4
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