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Progress in Modern Biomedicine. 2014, (28)

Tanshinone ⅡA Protect Hypoxic Myocardial Cells Through Regulating MicroRNA-1Chinese Full Text

LI Xue-lian;GUAN Hui-lin;WANG Lu;GUO Rui;ZHAO Dan-dan;GUO Zhen-feng;ZHANG Jia-lin;SHAN Hong-li;Department of Pharmacology,Harbin Medical University;State-Province Key Laboratories of Biomedicine-Pharmaceutics of China,Key Laboratory of Cardiovascular Research,Ministry of Education Heilongjiang;Mudanjiang Medical University;

Abstract: Objective: To study and explore the mechanism of Tanshinone ⅡA（Tan） protecting hypoxic myocardial cells by regulating microRNA-1. Methods: Primary cultured neonatal cardiomyocytes and established the model of myocardial hypoxia. Cell viability was detected by MTT assay, apoptosis of myocardial cells and apoptosis percentage were detected by TUNEL assay and flow cytometry. Intracellular calcium concentration（[Ca2+]i） was measured by Laser Scanning Confocal. Results: MTT results showed that the Tan ⅡA could alleviate cell death induced by hypoxia, with the trend of dose-dependence,（P＜0.05）. TUNEL results also showed that Tan ⅡA could significantly reduce the percentage of hypoxia-induced apoptosis（P＜0.05）. The [Ca2+]i increased significantly under hypoxia,while the Tan ⅡA could effectively inhibit the [Ca2+]i and thus play a protective role（P＜0.05）. Conclusions: Tanshinone ⅡA can alleviate the hypoxia-induced apoptosis, possibly through regulating miRNA-1 and [Ca2+]i, playing a protective effect on myocardial cells.
Keywords：

Tanshinone Ⅱ A; Hypoxia; Cardiomyocytes; miRNA-1; [Ca2+]i; Apoptosis;

  • DOI：

    10.13241/j.cnki.pmb.2014.28.016

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  • Classification Code：

    R285

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