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Progress in Modern Biomedicine. 2014, (18)

The Effect of Tanshinone on the Expression of iNOS, MMP-2 in Temporal of Alzheimer’s Disease Model Rats and Study on MechanismChinese Full Text

JIANG Ping;SUN Xiu-jia;XIANG Zheng-hua;SU Xiao-ping;LI Chun-bo;Shanghai Mental Health Center,Shanghai Jiao Tong University School of Medicine;College of Basic Medical Sciences,The Second Military Medical University;

Abstract: Objective: To investigate the influence of tanshinone II A（Tan II A） on the learning and memory ability, the expression of the inducible nitric oxide synthase（iNOS）, matrix metalloproteinase II（MMP-2） in temporal of Alzheimer’s disease（AD） model rats. Methods: AD model rats were established by direct β-amyloid protein（Aβ） injection method. For Tan II A intervention test, the learning and memory ability of AD rats were observed by passive avoidance task test, the expression of iNOS, MMP-2 at mRNA and protein levels in temporal region were compared by real-time PCR and Western Blot respectively. Using SPSS 13.0 to statistic analyse the data. Results: Compared with sham-operation group, the average latency time of passive avoidance task test in AD group was decreased（P＜ 0.01）, and the average wrong times of passive avoidance task test in AD group was significantly increased（P＜ 0.01）. Both mRNA and protein expressions of iNOS, MMP-2 in temporal were significantly increased（mRNA:P＜ 0.01, P＜ 0.01; protein: P＜0.01, P＜0.01）. Compared with AD group, the average latency time in Tan II A group was significantly increased（P＜0.01）, and the average wrong times in Tan II A group was significantly decreased（P＜0.01）. Both mRNA and protein expressions of iNOS, MMP-2 of temporal in Tan IIA group were significantly decreased respectively（mRNA:P＜0.05, P＜0.05; protein: P＜ 0.01, P＜ 0.01）. Conclusion: Tan II A can effectively enhance the ability of learning and memory of AD rats, significantly decrease mRNA and protein expressions of iNOS, MMP-2 in temporal of AD rats. This mechanism is probably inhibiting iNOS and MMP-2 expressions induced by Aβ in AD rats through suppressing oxidative injury.
Keywords：

Alzheimer’s disease; Nitric oxide synthase（iNOS）; Matrix metalloproteinase II（MMP-2）; Tanshinone II A;

  • DOI：

    10.13241/j.cnki.pmb.2014.18.001

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  • Subject：

  • Classification Code：

    R285.5

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