Influence of Low Molecular Weight Chondroitin Sulfate on IL-1β, TNF-αand TGF-β, Calcium and Phosphate in the Process of Cartilage Reconstruction of RabbitEnglish Full Text
PEI Tong;LIU Zhan-tao;GUO Xi-chun;LIU Kun;GAO Hua;School of Pharmaceutical Science, Qingdao university;
Abstract: Objective: To investigate the influence of low molecular weight chondroitin sulfate on IL-1β, TNF-α and TGF-β,calcium and phosphate in the process of cartilage reconstruction of rabbit. Methods: 36 New Zealand rabbits were randomly divided into6 groups with 6 rabbits in each group: control group, model group, low-dose of low molecular weight CS group, high-dose of low molecular weight CS group, low-dose of high molecular weight CS group and high-dose of high molecular weight CS group. A defect,3mm diameter, 3mm depth, was drilled at the condyles of femur of the hind legs by using an electric bone drill. After the next day for animal drug perfusion, 1 time a day. Drawn after 5 weeks. The expression of cytokines, such as IL-1β, TNF-α and TGF-β in synovial fluid was detected by ELISA, determining levels of calcium and phosphorus in serum of rabbit by automatic biochemical analyzer kit.Results: Low molecular weight and high molecular weight CS were able to reduce levels of inflammatory cytokines and increase levels of TGF-β in the joint fluid. And compared with that in the model group, there is statistical significance（P＜0.05）. While low molecular weight CS’ effect were better than high molecular weight CS（P＜0.05）. However, there was no obvious difference between high and low dose（P＞0.05）. Compared with that in the model group, the groups of CS, which levels of calcium and phosphorus in the serum were relatively low（P＜0.05）, but their levels were higher than that in the control group. However, these indexes had no obvious difference between low molecular weight CS group and high molecular weight CS. Conclusion: High and low molecular weight CS can increase the levels of TGF-β and reduce the levels of IL-1β, TNF-α and calcium, phosphate in the serum. For cartilage reconstruction, the above factors may have positive effect.
Keywords：
Low molecular weight of chondroitin sulfate; Cartilage reconstruction; IL-1β; TNF-α; Phosphate;
- DOI：
10.13241/j.cnki.pmb.2014.17.013
- Series：
- Subject：
- Classification Code：
R965
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