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Environmental Science. 2014, (08) Core

Isolation,Identification and Characterization of a Diethylstilbestrol-degrading Bacterial Strain Serratia sp.Chinese Full Text

XU Ran-fang;SUN Min-xia;LIU Juan;WANG Hong;LI Xin;ZHU Xue-zhu;LING Wan-ting;Institute of Organic Contaminant Control and Soil Remediation,Nanjing Agricultural University;

Abstract: Utilizing the diethylstilbestrol（DES）-degrading bacteria to biodegrade DES is a most reliable technique for cleanup of DES pollutants from the environment. However,little information is available heretofore on the isolation of DES-degrading bacteria and their DES removal performance in the environment. A novel bacterium capable of degrading DES was isolated from the activated sludge of a wastewater treatment plant. According to its morphology,physiochemical characteristics,and 16 S rDNA sequence analysis,this strain was identified as Serratia sp.. The strain was an aerobic bacterium,and it could degrade 68. 3% of DES（50 mg·L- 1） after culturing for 7 days at 30℃,150 r·min- 1in shaking flasks. The optimal conditions for DES biodegradation by the obtained strain were 30℃,40-60 mg·L- 1DES,pH 7. 0,5% of inoculation volume,0 g·L- 1of added NaCl,and 10 mL of liquid medium volume in 100 mL flask.
Keywords：

diethylstilbestrol（DES）; estrogenic hormone; identification; biodegradation; Serratia sp.;

  • DOI：

    10.13227/j.hjkx.2014.08.047

  • Series：

  • Subject：

  • Classification Code：

    X172

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