Screening,identification and growth characteristics of soil organic phosphate degrading bacterial strainsChinese Full Text
SUN Wei;GU Jie;LI Yu-di;QIAN Xun;WANG Xiao-juan;Collage of Natural Resource and Environment,Northwest A＆F University;Research Center of Recycle Agricultural Engineering and Technology of Shaanxi Province;
Abstract: 【Objective】This study screened organic-phosphate degrading bacteria from soil,identified the bacteria,studied the physiological and biochemical characteristics to provide efficient and stable strains with ability of degrading soil organic phosphate as bio-organic fertilizers.【Method】Using degrading circle method and liquid shake flask culture method,phytin and lecithin were used as the only phosphate source to screen bacteria from soil sample collected from various locations（the outfall of pesticide factory,cotton field,orchard and vegetable plot）in Yangling,Shaanxi and molecular biological method was utilized for identification.The proper temperature and initial pH for strains were investigated as well.【Result】25organic-phosphate degrading bacteria were screened from soil samples.Z2-3,Z3-5,Z3-8,and Z4-1had high phytin degrading rates,and L5-2and L7-1had high lecithin degrading rates after recreening.The phytin degrading rates of Z2-3,Z3-5,Z3-8and Z4-1in liquid medium reached 64.2%,71.5%,67.2% and 58.1%, while lecithin degrading rates of L5-2and L7-1in liquid medium were 76.7% and 84.1%,respectively. Physiological and biochemical characteristics and 16SrDNA analysis demonstrated that Z2-3,Z3-5,Z3-8, Z4-1,L5-2and L7-1were Pseudomonas plecoglossicida,Pseudomonas putida,Pseudomonas fluorescens, Pseudomonas fluorescens,Pantoea agglomerans and Brevibacillus agri,respectively.The most suitable culture temperatures of phytin degrading bacteria and lecithin degrading bacteria were 28-35 ℃ and 28 ℃,respectively,and the most suitable initial pH values of phytin degrading bacteria and lecithin degrading bacteria were 8.0-8.5and 7.5,repsectively.【Conclusion】6strains with high organic-phosphorus degrading rates were isolated and identified.The most suitable culture temperatures and initial pH values were obtained as well.
Keywords：
organic-phosphate degrading bacteria; isolation and identification; identification; growth characteristics; Phytin; lecithin;
- DOI：
10.13207/j.cnki.jnwafu.2014.02.058
- Series：
- Subject：
- Classification Code：
X172
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