Chinese Journal of Biologicals. 2014, (06)

Preparation and immune effect of meningococcal immunogenic conjugatesChinese Full Text

ZHANG Ying;ZHANG Shui-hua;LI Zhi-long;WANG Yan;School of Applied Chemistry and Biotechnology,Shenzhen Polytechnic;

Abstract: Objective To conjugate Neisseria meningitidis（Nm）groupsA,B,W135 and Y by using various carriers,and compare the immune effects of conjugates. Methods The polysaccharides of Nm groups A,W135 and Y were conjugated to CRM197. The seeds of Nm group B were subcultured,from which outer membrane protein was extracted,purified and conjugated to polysaccharide group C. The protein-polysaccharide conjugates were mixed with liposome by freezedried hydratation and immunized to guinea pigs,using alum as control,and determined for bactericidal titer. Results The conjugates showed various immune effects against Nm of the corresponding serogroups. Compared with that of direct mixture,the covalent conjugate of polysaccharide group C and outer membrane protein group B induced high bactericidal titer in guinea pigs. The immunopotentiation of conjugates using liposome as adjuvant was superior to that using alum as control,while the immune effects of conjugates groups A and Y were enhanced significantly（P ＜ 0. 05）. Conclusion The immunogenic conjugates of Nm prepared with various antigens by covalent conjugation showed higher bactericidal titer than those by direct mixing. In addition,liposome as an adjuvant was beneficial to the enhancement of immune effect of Nm vaccine.

Keywords：

Neisseria meningitidis（Nm）; Carrier protein; Conjugation; Liposome; Bactericidal titer;

DOI：
10.13200/j.cnki.cjb.000397
Series：
Subject：
Classification Code：
R515.2

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Preparation and immune effect of meningococcal immunogenic conjugatesChinese Full Text

ZHANG Ying;ZHANG Shui-hua;LI Zhi-long;WANG Yan;School of Applied Chemistry and Biotechnology,Shenzhen Polytechnic;

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