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Chinese Archives of Traditional Chinese Medicine. 2010, (02)

Effects of Macrocalyxin A on Kasumi-1 Leukemia Cells in VitroChinese Full Text

WANG Zhen-ni1,2,ZHOU Yong-lie2,LU Ya-ping3,XIA Jun2,SHI Hao3,QIU Lian-nu1,LI Wei-xing2(1.Zhejiang Provincial People’s Hospital,Hangzhou 310014,Zhengjiang,China;2.Zhejiang Chinese Medical University,Hangzhou 310054,Zhengjiang,China;3.The Medical College of Zhejiang Industry University,Hangzhou 310004,Zhengjiang,China)

Abstract: Objective: To investigate the effects of Macrocalyxin A inducing apoptosis and proliferation on kasumi-1 leukemia cells in vitro.Methods: Kasuni-1 cells in vitro were treated with different concentrations（4,8,12,16μg/mL） of MA,The inhibitory rate of the cells was measured by MTT assay and alive cell count;differentiation of Kasumi-1 cells were evaluated by cell morphology and Differentiation-related antigens of myelocyte as CD11b and CD13;morphology of apoptosis was observed by Hoechst 33258 fluorescence staining and DNA fragmentation was assayed by agarosegelelectrophoresis.;cell apoptotic rate was detected by DNA subdiploid content,Annexin-V/PI stainning;SELDI-TOF mass spectrometry was used to detect the protein fingerprinting of cell after different concerntrations of MA culture.Results: MA significantly inhibited the kasumi-1cell growth in a time-and dose-dependent.Specially,the expression of CD11b obviously increased in a dose-dependent manner.which indicated low dose MA induced cell differentiation.Meanwhile,significant increase of sub-G1 cells and annexinV-FITC+ cell showed a dose-dependent manner comparing with the control cells and Hoechst 33258 fluorescence staining showed characteristic apoptostic body,DNA fragmentation can be observed in kasumi-1cell treated with MA.Moreover,there were thirteen different protein peaks by mass spectrometryand the M/Z values range from 1053 to 8581.Conclusion: MA can inhibit proliferation and induced apoptosis and differentiation of Kasumi-1 cell.
Keywords：

macrocalyxin A（MA）; Kasumi-1 cell; Apoptosis; Spectrometry;

  • DOI：

    10.13193/j.archtcm.2010.02.85.wangzhn.041

  • Series：

  • Subject：

  • Classification Code：

    R285

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