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Liaoning Journal of Traditional Chinese Medicine. 2011, (12) Core

Senescence and Influence of Human Umbilical Vein Endothelial Cells Induced by Different Concentrations Angiotensin ⅡChinese Full Text

WANG Xue-ni1,2,LIU Ling1,LIU Ze1,WANG Lu-ni1,WU Jun1,WANG Wei1,FENG Ju-xiang1(1.Guangzhou General Hospital of Guangzhou Military Command,Guangzhou 510010,Guangdong,China; 2 Guangzhou University of Chinese Medicine,Guangzhou 510405,Guangdong,China)

Abstract: Objective:To investigate the changes of the survival rate of Human umbilical vein endothelial cells（HUVECs）aging induced by angiotensin Ⅱ（AngⅡ） at different concentrations.Methods:HUVECs were cultured in vitro and designed randomly 5 groups as follows:control group,different concentrations groups（10-8~10-5mmol/L）.HUVECs were treated with AngII at concentrations of 0mmol/L,10-8mmol/L,10-7mmol/L,10-6mmol/L,10-5mmol/L respectively for 48h.β-gal staining was used to identify cell aging status;Flow cytometry was used for analyzing cell cycle;the cell living rate was separately observed by MTT and CCK-8.Results:After incubating the HUVECs with AngⅡ at different concentrations for 48h,the positive cell number of β-gal staining was gradually higher with the increasing concentrations（P＜0.01）;the cell cycle was arrested at G0/G1 phase,the relative percentage of S phase was decreased gradually;the cell living rate by AngII-induced obviously declined compared with control group;there was no significant difference in the concentration of 10-8 and 10-7mmol/L groups（P＞0.05）;however,10-6 and 10-5mmol/L groups has statistically significant difference（P＜0.05）.Conclusion:AngII induced HUVECs senescence and inhibited the cell proliferative activity according to the AngII concentrations.It means the increasing AngII concentrations could accelerate cell aging.
Keywords：

Angiotensin Ⅱ; HUVECs; Cell senescence;

  • DOI：

    10.13192/j.ljtcm.2011.12.175.wangxn.072

  • Series：

  • Subject：

  • Classification Code：

    R363

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