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Chinese Heart Journal. 2008, (05)

Effect of orphan nuclear receptor Nur77 on inflammatory factor secretion in murine macrophage strainChinese Full Text

HU Liu-hua1,HE Ben1,SHEN Ling-hong1,JIANG Li-sheng1,CHAI Da-jun1,WANG Li2,ZENG Jin-zhang2 (1.Department of Cardiology,Renji Hospital,School of Medicine,Shanghai Jiaotong University,Shanghai 200127,China;2.SAMOST GROUP Union R ＆ D Center,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,Shanghai 200233,China)

Abstract: AIM To observe the effect of orphan nuclear receptor Nur77 on inflammatory factor secretion in murine macrophage RAW264.7 induced by oxidized low density lipoprotein（ox-LDL）.METHODS RAW264.7 cells stably expressing transfecting plasmid GFP-Nur77 and GFP cDNA（as a control） were respectively established.After the two transfected macrophages were incubated with ox-LDL（40 mg/L）,the concentrations of TNF-α,IFN-γ,MMP-9,MCP-1 and IL-6 in culture supernatant were detected by ELISA.RESULTS After the ox-LDL stimulation,the concentrations of IFN-γ,TNF-α,MMP-9 and MCP-1 in the two transfected macrophages all increased（P＜0.05）.However,the increased concentrations in GFP-Nur77-RAW264.7 were significantly less than those in GFP-RAW264.7（P＜0.05）.Before ox-LDL stimulation,the concentrations of TNF-α,IFN-γ,MMP-9 and Mcp-1 in GFP-RAW264.7 were respectively（19.00±1.69） ng/L,（99.10±3.09） ng/L,（0.14±0.02） ng/L and（23.96±3.57） ng/L.After the stimulation,the concentrations of the 4 inflammatury factors increased to（27.22±0.38） ng/L,（549.90±1.81） ng/L,（0.45±0.06） ng/L and（49.31±3.66） ng/L respectively.Before ox-LDL stimulation,the concentrations of the 4 inflammatory factors secreted by GFP-Nur77-RAW264.7 and GFP-RAW264.7 had no statistical difference.After ox-LDL stimuladtion,the concentrations of the 4 inflammatory factors increased to（18.34±1.06） ng/L,（320.56±1.27） ng/L,（0.20±0.03） ng/L and（38.92±4.46） ng/L respectively.However,no significant changes were observed in IL-6 concentration secreted by GFP-Nur77-RAW264.7 or GFP-RAW264.7.CONCLUSION High expression of Nur77 on RAW264.7 cells obviously reduce the inflammatory factor secretion induced by ox-LDL.This may be an important protective mechanism of Nur77.
Keywords：

orphan nuclear receptor; ox-LDL; macrophage; inflammatory factor; mouse;

  • DOI：

    10.13191/j.chj.2008.05.35.hulh.027

  • Series：

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  • Classification Code：

    R543.5

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