Effects of Fenofibrate on cardiomyocyte apoptosis and expression of apoptosis-associated gene Fas and Fas-L in pressure overload ratsChinese Full Text

WU Qiang1,YANG Yong-yao1,LI Long-gui2,YANG Tian-he1,CAI Yun-chang1,JIANG Qing-an1 (1.Department of Cardiology,Guizhou Province People′s Hospital,Guiyang 550002,Guizhou,China;2.Department of Cardiology,Xinqiao Hospital,Third Military Medical University,Chongqing 400037,China)

Abstract: AIM To investigate the effects of ligands of PPARα fenofibrate on cardiomyocyte apoptosis and expression of apoptosis-associated gene,Fas and Fas ligand（Fas-L） in pressure overload rats.METHODS A pressure overloading model was established by abdominal aorta constriction in Wistar rats.Forty-eight hours after constriction,the surviving rats were randomly divided into 4 groups: 4-week coarctation of abdominal aorta group,8-week coarctation of abdominal aorta group,4-week Fenofibrate [30 mg/（kg·d）] treatment group and 8-week Fenofibrate treatment group.Additional 10 rats that underwent abdominal cavity incision without ligation served as sham group.After 4 weeks and 8 weeks of therapy,the ratio of left ventricular weight to body weight（LVW/BW） and the ratio of right ventricular weight to body weight（RVW/BW） were measured.DNA fragmentations were determined semiquantitatively by in situ TDT-mediated dUTP nick end labeling（TUNEL）.Using Western blot,Fas and Fas-L expression was detected.RESULTS Fenofibrate treatment for 8 week significantly reduced LVW/BW,cardiomyocyte apoptosis and expression of Fas and Fas-L in pressure overload rats.In contrast,there was no significant difference in the above mentioned indices between the group with aortic constriction and the group treated with 4-week Fenofibrate.CONCLUSION Chronic treatment（8 weeks） with the PPARα agonist may be useful in preventing cardiac hypertrophy and apoptosis.The proto-oncogene Fas and Fas-L play a key role in PPARα pathway,regulating cardiocyte apoptosis.

Keywords：

peroxisome proliferator-activated receptors; fenofibrate; apoptosis; pressure overload; gene;

DOI：
10.13191/j.chj.2007.05.12.wuq.013
Series：
Subject：
Classification Code：
R541

Mobile Reading

Read on your phone instantly

Step 1
Scan QR Codes

"Mobile CNKI-CNKI Express" App

Step 2
Open“CNKI Express”

and click the scan icon in the upper left corner of the homepage.

Step 3
Scan QR Codes

Read this article on your phone.
HTML
CAJ Download
PDF Download

Contents

Effects of Fenofibrate on cardiomyocyte apoptosis and expression of apoptosis-associated gene Fas and Fas-L in pressure overload ratsChinese Full Text

WU Qiang1,YANG Yong-yao1,LI Long-gui2,YANG Tian-he1,CAI Yun-chang1,JIANG Qing-an1 (1.Department of Cardiology,Guizhou Province People′s Hospital,Guiyang 550002,Guizhou,China;2.Department of Cardiology,Xinqiao Hospital,Third Military Medical University,Chongqing 400037,China)

Read on your phone instantly

Citation Network

Related Literature