Effects of Simvastatin on LPS induced NO production and AT2R expression in rat cardiac fibroblastsChinese Full Text

LIU Shao-wei, ZHAO Lian-you, Zheng Qiang-sun, SHANG Fu-jun, ZHANG Li-juan, LIU Hui, HE Yan-ping, ZHAO Xiao-yan(Department of Cardiology, Tangdu Hospital, Fourth Military Medical University, Xi′an 710038, Shaanxi, China)

Abstract: AIM To investigate the effects of Simvastatin （Sim） on lipopolysaccharides （LPS）-induced changes in nitric oxide （NO） production and angiotensin Ⅱ type 2 receptor （AT2R） expression in cardiac fibroblasts （CFs）. METHODS First cultures of neonatal rat CFs were employed in the present study. After incubating cells with LPS, the production of intrinsic nitric oxide （NO） was determined using nitrate reductase measurement. The protein levels of AT2R was determined by Western blot analysis. RESULTS No significant difference was observed in NO production and AT2R protein expression （shown as AT2R/β-actin） when CFs were incubated with Sim （0.01 μmol/L） plus LPS （10 mg/L） or Sim （0.1 μmol/L） plus LPS （10 mg/L） and incubated with LPS alone. NO production increased significantly when CFs were incubated with 1 μmol/L or 10 μmol/L Sim in the presence of LPS, compared with LPS alone （both P＜0.01）, while AT2R/β-actin decreased significantly in the coincubated groups （P＜0.05 and P＜0.01, respectively）. CONCLUSION High concentrations of Sim antagonize LPS in the induction of NO release and the inhibition of AT2R protein expression.

Keywords：

statins; cardiac fibroblasts; nitric oxide; angiotensin Ⅱ type 2 receptor; apoptosis;

DOI：
10.13191/j.chj.2007.03.10.liushw.003
Series：
Subject：
Classification Code：
R96

Mobile Reading

Read on your phone instantly

Step 1
Scan QR Codes

"Mobile CNKI-CNKI Express" App

Step 2
Open“CNKI Express”

and click the scan icon in the upper left corner of the homepage.

Step 3
Scan QR Codes

Read this article on your phone.
HTML
CAJ Download
PDF Download

Contents

Effects of Simvastatin on LPS induced NO production and AT2R expression in rat cardiac fibroblastsChinese Full Text

LIU Shao-wei, ZHAO Lian-you, Zheng Qiang-sun, SHANG Fu-jun, ZHANG Li-juan, LIU Hui, HE Yan-ping, ZHAO Xiao-yan(Department of Cardiology, Tangdu Hospital, Fourth Military Medical University, Xi′an 710038, Shaanxi, China)

Read on your phone instantly

Citation Network

Related Literature