Mesenchymal stem cells differentiate into interstitially seeded cells for tissue engineering of heart valvesChinese Full Text

ZHANG Jin-bao,GU Chun-hu,LIU Wei-yong,WANG Yun-ya,WEI Xiang-yang,OUYANG Hui,LIU Xing-guang(Department of Cardiovascular Surgery,Xijing Hospital,Fourth Military Medical University,Xi′an,Shaanxi 710032,China)

Abstract: AIM To investigate differentiation of mesenchymal stem cells into interstitially seeded cells of tissue engineering heart valves.METHODS The canine bone mesenchymal stem cells（BMSCs） were separated,expanded and differentiated into interstitial cells.The induced BMSCs were cultured,expanded,purified,frozen and resuscitated.Compared with venous mesenchymal cells,the bone marrow-derived fibroblasts were examined in cell morphology,proliferation capability,collagen-synthesized capability and adhesive onto detergent acellularized porcine valves.The capability of cell resuscitating was studied between the two kinds of mesenchymal cells.RESULTS Both BMSCs and bone marrow-derived fibroblasts were adhensive growth cells and were spindle in shape.The double time of the bone marrow-derived interstitial cells and saphenous interstitial cells was about 38 h and 36 h,respectively.There was no significant difference in collagen-synthesized capability and cell resuscitating rate.The two kinds of cells could be planted on detergent acellularized porcine-valves by scanning electron microscope and interstitial cells were found on the scaffold by immuohistochemistry method.CONCLUSION BMSCs can be induced to differentiate into interstitial cells and can be used as seeded cells to fabricate tissue engineered heart valves.

Keywords：

mesenchymal stem cells; Cell differentiation; interstitial cells; seeded cells; tissue engineering;

DOI：
10.13191/j.chj.2006.05.13.zhangjb.003
Series：
Subject：
Classification Code：
R318

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Mesenchymal stem cells differentiate into interstitially seeded cells for tissue engineering of heart valvesChinese Full Text

ZHANG Jin-bao,GU Chun-hu,LIU Wei-yong,WANG Yun-ya,WEI Xiang-yang,OUYANG Hui,LIU Xing-guang(Department of Cardiovascular Surgery,Xijing Hospital,Fourth Military Medical University,Xi′an,Shaanxi 710032,China)

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