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Chinese Heart Journal. 2005, (05)

An experimental technique for cellular cardiomyoplastyChinese Full Text

WEN Jun~(1),LIU Lu-wei~(2),FAN Yun~(1),QU Xue-min~(1),LONG Kai-ping~(1),WANG Si-gang~(1)((1.Department) of Physics,(2.Out-patient) department,Fourth Military Medical University,Xi′an,Shaanxi 710033,China)

Abstract: AIM: To explore a new experimental surgical technique for the treatment of heart failure.METHODS: A newly designed apparatus for stabilizing a beating mouse heart was applied with a built-in channel through which prepared cell suspension was injected into the cryoinjured area of left ventricles.A transgenic mouse model was used to provide the special cells for the injection.RESULTS: The number of green luorescent protein（GFP）-labeled cardiomyocytes of the injured myocardium using the new technique was significantly higher than using syringes for cell injection（（P＜0.01））.（CONCLUSION）: The new technique significantly increases ostoperative survival rate.
Keywords：

mouse; surgery,experiment; technique; cardiomyocytes;

  • DOI：

    10.13191/j.chj.2005.05.45.wenj.014

  • Series：

  • Subject：

  • Classification Code：

    R-331

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