Effect of bFGF on free Ca2+ intracellular and calcification of canine saphenous vein interstitial cells（SVICs） cultured in vitroChinese Full Text
SU Xue-jia,LIU Wei-yong,CHEN Wen-sheng,GU Chun-hu,OU Yang-hui,WEI Xiang-yang(Department of Cardiovascular Surgery,Xijing Hospital,Fourth Military Medical University,Xi’an,Shaanxi 710032,China)
Abstract: AIM:To observe the effect of bFGF on intracellular free Ca2+ and calcification of saphenous vein interstitial cells（SVICs） as seed cells of tissue engineering valve cultured in vitro.METHODS: Canine SVICs were obtained and identified with immunocytochemistry.Cells of passage 3~6 were devided into the control and bFGF groups.In the control group,cells were cultured in standard medium.In the bFGF group,cells were cultured in medium with 5 μg/L bFGF.After 2 weeks,free Ca2+ and cellular apoptosis rate were determined by laser confocal microscope and flow cytometry,respectively.The cellular layer Calcium deposition was measured by atomic absorption spectrometry and stained by von kossa.（RESULTS）: ①SVICs were mainly composed of smooth muscle cells,myofibroblasts and fibroblasts.② In the control group,cellular nodules formed and calcification was mild positive by von kossa staining.In the bFGF group,cells grew with dense polarity;cellular nodules did not form and calcification was negative.③ Intracellular free Ca2+ concentration in the control group was higher than that in the bFGF group.Cellular apoptosis rate and calcifical deposition in the bFGF group were significantly lower than those in the control group.CONCLUSION: bFGF could maintain SVICs [Ca2+]i homeostasis and inhibit cell apoptosis and calcification.
Keywords：
tissue engineering valve; interstitial seed cells; bFGF; intracellular Ca2+; calcification;
- DOI：
10.13191/j.chj.2005.05.34.suxj.009
- Series：
- Subject：
- Classification Code：
R318.0;
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