Mucopolysaccharides concentrations in blood and urine and some enzyme activities in their metabolism were determined in cattle with laminitis. Acid mucopolysaccharides (AMPS) concentrations in blood and urine and the activity of blood N-acetyl-β-D-glucosaminidase were higher in the affected cattle than in controls, suggesting the abnormal metabolism of mucopolysaccharides in the hoofs with laminitis.