Expression and Subcellular Localization of E-Cadherin, α-Catenin, and β-Catenin in 8 Feline Mammary Tumor Cell Lines

Shun-roku TAKAUJI; Manabu WATANABE; Rina UYAMA; Takayuki NAKAGAWA; Nozomi MIYAJIMA; Manabu MOCHIZUKI; Ryohei NISHIMURA; Sumio SUGANO; Nobuo SASAKI

doi:10.1292/jvms.69.831

NOTE

Expression and Subcellular Localization of E-Cadherin, α-Catenin, and β-Catenin in 8 Feline Mammary Tumor Cell Lines

Shun-roku TAKAUJI, Manabu WATANABE, Rina UYAMA, Takayuki NAKAGAWA, Nozomi MIYAJIMA, Manabu MOCHIZUKI, Ryohei NISHIMURA, Sumio SUGANO, Nobuo SASAKI

Author information

Shun-roku TAKAUJI
Laboratory of Veterinary Surgery, Graduate School of Agricultural and Life Sciences
Manabu WATANABE
Laboratory of Functional Genomics, Department of Medical Genome Sciences, Graduate School of Frontier Sciences, The University of Tokyo,
Rina UYAMA
Laboratory of Veterinary Surgery, Graduate School of Agricultural and Life Sciences
Takayuki NAKAGAWA
Laboratory of Veterinary Surgery, Graduate School of Agricultural and Life Sciences
Nozomi MIYAJIMA
Laboratory of Veterinary Surgery, Graduate School of Agricultural and Life Sciences
Manabu MOCHIZUKI
Laboratory of Veterinary Surgery, Graduate School of Agricultural and Life Sciences
Ryohei NISHIMURA
Laboratory of Veterinary Surgery, Graduate School of Agricultural and Life Sciences
Sumio SUGANO
Laboratory of Functional Genomics, Department of Medical Genome Sciences, Graduate School of Frontier Sciences, The University of Tokyo,
Nobuo SASAKI
Laboratory of Veterinary Surgery, Graduate School of Agricultural and Life Sciences

Keywords: α- and β-catenin, E-cadherin, feline mammary tumor

JOURNAL FREE ACCESS

2007 Volume 69 Issue 8 Pages 831-834

DOI https://doi.org/10.1292/jvms.69.831

Details

Abstract

Protein expression and subcellular localization of E-cadherin, α-catenin, and β-catenin in 8 feline mammary tumor cell lines were examined by western blot analysis and fluorescence immunocytochemistry. A low E-cadherin expression was observed in FNN-m cells. Furthermore, compared to other cell lines, two E-cadherin bands existed in FMC-p1 cells and were localized in the perinuclear region; distinct radial lines were observed in the cytoplasm. A low α-catenin expression was observed in FON-m cells, but there were no apparent abnormalities in its localization. In contrast, similar levels of β-catenin expression and cytoplasmic localization were observed in all cell lines.

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