Gene Expression Analysis of Peroxisome Proliferators- and Phenytoin-Induced Hepatotoxicity Using cDNA Microarray

Jin-Wook JUNG; Joon-Suk PARK; Jae-Woong HWANG; Kyung-Sun KANG; Yong-Soon LEE; Bog-Soo SONG; Gyoung-Jae LEE; Chan-Dong YEO; Jong-Soo KANG; Wan-Seon LEE; Ki-Seon JEON; Chan-Hwi UM; Yang-Suk KIM; Moon-Ju OH; Jong-Pil YOUN; Peng LI; Jung-Eun PARK; Seung Yong HWANG

doi:10.1292/jvms.66.1329

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Gene Expression Analysis of Peroxisome Proliferators- and Phenytoin-Induced Hepatotoxicity Using cDNA Microarray

Jin-Wook JUNG, Joon-Suk PARK, Jae-Woong HWANG, Kyung-Sun KANG, Yong-Soon LEE, Bog-Soo SONG, Gyoung-Jae LEE, Chan-Dong YEO, Jong-Soo KANG, Wan-Seon LEE, Ki-Seon JEON, Chan-Hwi UM, Yang-Suk KIM, Moon-Ju OH, Jong-Pil YOUN, Peng LI, Jung-Eun PARK, Seung Yong HWANG

Author information

Jin-Wook JUNG
Department of Biochemistry and Molecular Biology, Hanyang University & Genocheck Co., Ltd.
Joon-Suk PARK
Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University
Jae-Woong HWANG
Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University
Kyung-Sun KANG
Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University
Yong-Soon LEE
Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University
Bog-Soo SONG
Research Institute. Shin-Won Scientific Co., Ltd. #702 Kowoon Institute of Technology Innovation, Suwon University
Gyoung-Jae LEE
Research Institute. Shin-Won Scientific Co., Ltd. #702 Kowoon Institute of Technology Innovation, Suwon University
Chan-Dong YEO
Research Institute. Shin-Won Scientific Co., Ltd. #702 Kowoon Institute of Technology Innovation, Suwon University
Jong-Soo KANG
Research Institute. Shin-Won Scientific Co., Ltd. #702 Kowoon Institute of Technology Innovation, Suwon University
Wan-Seon LEE
Bioimformatics Unit, ISTECH Inc.
Ki-Seon JEON
Bioimformatics Unit, ISTECH Inc.
Chan-Hwi UM
Bioimformatics Unit, ISTECH Inc.
Yang-Suk KIM
Bioimformatics Unit, ISTECH Inc.
Cancer Metastasis Research Center, Yonsei University College of Medicine
Moon-Ju OH
Department of Biochemistry and Molecular Biology, Hanyang University & Genocheck Co., Ltd.
Jong-Pil YOUN
Department of Biochemistry and Molecular Biology, Hanyang University & Genocheck Co., Ltd.
Peng LI
Department of Biochemistry and Molecular Biology, Hanyang University & Genocheck Co., Ltd.
Jung-Eun PARK
Department of Biochemistry and Molecular Biology, Hanyang University & Genocheck Co., Ltd.
Seung Yong HWANG
Department of Biochemistry and Molecular Biology, Hanyang University & Genocheck Co., Ltd.

Keywords: gene expression, microarray, peroxisome proliferators, phenytoin, toxicogenomics

JOURNAL FREE ACCESS

2004 Volume 66 Issue 11 Pages 1329-1333

DOI https://doi.org/10.1292/jvms.66.1329

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Abstract

The recent DNA microarray technology enables us to understand a large number of gene expression profiling. The technology has potential possibility to comprehend mechanism of multiple genes were related to compounds which have toxicity in biological system. So, the toxicogenomics through this technology may be very powerful for understanding the effect of unknown toxic mechanisms in biological system. We have studied that the effect of compounds related to hepatotoxin in vivo system using DNA microarray and classified chemicals which have been well characterized. We have studied three compounds; 2 peroxisome proliferators: Clofibrate (ethyl-p-chlorophenoxyisobutyrate), gemfibrozil (5-2[2,5-dimethyl-phenoxy]2-2-dimethyl-pentanonic), and an antiepileptic drug: phenytoin (5,5-diphenylhydantoin). Male Sprague-Dawely VAF⁺ albino rats of 5-6 weeks old were treated with each compound for 24 hr and 2 weeks. 4.8 K cDNA microarray in house has been used for gene expression profiling. We found that the clustering of gene expression had similarity like as the toxic phenotype of compounds.

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