The purpose of this study is to attempt the induction of early immunopotentiation of antibodies specific to fimbriae of Salmonella enterica serovar Enteritidis(SE), by administering thymulin and zinc to SE−vaccinated chicken breeders, and the improvement of protection against a controlled−live challenge by SE.The first two groups of breeders were administered subcutaneously at 15 and 19 weeks of age a killed SE vaccine.Breeders of the third and fourth groups were left unvaccinated.Breeders of the first group, immunopotentiated by thymulin and zinc, were able to induce the earliest antibodies in their pooled sera at 2 weeks post the first SE−vaccination, specific to fimbriae(21 KDa)of SE.However, the second group that was only vaccinated with the same SE−vaccine produced specific antibodies to fimbriae at 3 weeks following the second vaccination(22 weeks of age).Breeders of the third group, that were neither SE−vaccinated nor immunopotentiated by thymulin and zinc, but were challenged by live SE at 22 weeks of age, were able to show specific antibodies to fimbriae at 3 weeks post challenge(25 weeks of age).The fourth group that was deprived of SE−vaccination, immunopotentiators, and challenge didn’t show any background antibodies specific to SE−fimbriae.The presence of the earliest antibody−immunopotentiation to fimbriae of SE in breeders of the first group, administered thymulin and zinc, was associated with the lowest frequency of SE−infected ceca(10%)among the challenged groups.In addition, breeders of the first group were the only challenged birds resulting in absence of SE infection in their cecal tonsils.The first group−vaccinated, immunopotentiated, and challenged, and the second group−vaccinated and challenged only resulted in breeders with absence of SE infection in their oviducts and spleens.In conclusion, immunopotentiation of chicken breeders by thymulin and zinc induces the earliest specific antibodies to fimbriae of SE associated with the lowest frequency of SE−infected ceca, and absence of SE infection from cecal tonsils, oviducts and spleens.