The Complete Amino Acid Sequence of Dog β2-Microglobulin

Yuichi Nakajima; Fumio Hoshi; Seiichi Higuchi; Seiichi Kawamura

doi:10.1292/jvms.61.517

Abstract

Dog β₂-microglobulin was purified from the urine of dogs with potassium dichromate induced tubular damage. It was purified by sequential use of anion exchange chromatography, gel filtration chromatography, and reversed-phase high performance liquid chromatography. Comparisons of the amino acid sequence of the dog protein with human, mouse, and rabbit β₂-microglobulin, indicated a high degree of similarity. The dog protein was very similar to human β₂-microglobulin in that it had a molecular weight of 11.8 kDa and contained two half-cystinyl residues. Dog and human β₂-microglobulin were demonstrably different at 24 of the 99 positions compared. The data supported the conclusion that the purified protein was dog β₂-microglobulin and that all four proteins from dog, human, mouse, and rabbit were closely related.

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この記事はクリエイティブ・コモンズ [表示 - 非営利 - 改変禁止 4.0 国際]ライセンスの下に提供されています。
https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja

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