Molecular Cloning of Feline Interleukin-6 cDNA

Takashi OHASHI; Yasunobu MATSUMOTO; Toshihiro WATARI; Ryo GOITSUKA; Hajime TSUJIMOTO; Atsuhiko HASEGAWA

doi:10.1292/jvms.55.941

Takashi OHASHI, Yasunobu MATSUMOTO, Toshihiro WATARI, Ryo GOITSUKA, Hajime TSUJIMOTO, Atsuhiko HASEGAWA

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Keywords: cDNA interleukin-6, feline, polymerase chain reaction

JOURNAL FREE ACCESS

1993 Volume 55 Issue 6 Pages 941-944

DOI https://doi.org/10.1292/jvms.55.941

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Abstract

We cloned and sequenced feline interleukin-6 (IL-6) cDNA using the polymerase chain reaction (PCR) technique to obtain information on regulation of the IL-6 gene. The feline IL-6 cDNA cloned in this study was 752 bp long and included all the amino acid coding region. At the amino acid level, the overall identities of the feline IL-6 to the human and murine counterparts were 58% and 39%, respectively. After transfection with the expression vector containing the cDNA, CRFK cells produced biologically active proteins that showed hybridoma growth promoting activity.

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