Diversity of Chlamydiales detected in pet birds privately kept in individual homes in Japan

Yukiko SASSA-O’BRIEN; Kenji OHYA; Shunsuke IKEUCHI; Naoko SANADA; Yasuyuki SANADA; Atsushi KOJIMA; Mario YORISAKI; Kazumasa EBISAWA; Kengo UEDA; Haruko IKI; Masaya MIZUKAMI; Hideki HAYASHIDANI; Hideto FUKUSHI

doi:10.1292/jvms.23-0185

Bacteriology

Diversity of Chlamydiales detected in pet birds privately kept in individual homes in Japan

Yukiko SASSA-O’BRIEN, Kenji OHYA, Shunsuke IKEUCHI, Naoko SANADA, Yasuyuki SANADA, Atsushi KOJIMA, Mario YORISAKI, Kazumasa EBISAWA, Kengo UEDA, Haruko IKI, Masaya MIZUKAMI, Hideki HAYASHIDANI, Hideto FUKUSHI

Author information

Yukiko SASSA-O’BRIEN
Laboratory of Veterinary Infectious Disease, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Tokyo, Japan
Kenji OHYA
Laboratory of Veterinary Microbiology, Faculty of Applied Biological Sciences, Gifu University, Gifu, Japan
Present address: National Institute of Health Sciences, Kanagawa, Japan
Shunsuke IKEUCHI
Laboratory of Veterinary Hygiene, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Tokyo, Japan
Naoko SANADA
Bird’s Hospital-BIRD HOUSE, Chiba, Japan
Yasuyuki SANADA
Bird’s Hospital-BIRD HOUSE, Chiba, Japan
Atsushi KOJIMA
Little Bird Hospital, Tokyo, Japan
Mario YORISAKI
Morishita Bird Clinic, Tokyo, Japan
Kazumasa EBISAWA
Yokohama Bird Clinic, Kanagawa, Japan
Kengo UEDA
Vogel Animal Hospital, Hyogo, Japan
Haruko IKI
Kalmia Bird Hospital, Aichi, Japan
Masaya MIZUKAMI
Mizukami Veterinary Hospital, Dogs, Cats and Birds, Kanagawa, Japan
Hideki HAYASHIDANI
Laboratory of Veterinary Hygiene, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Tokyo, Japan
Hideto FUKUSHI
Laboratory of Veterinary Microbiology, Faculty of Applied Biological Sciences, Gifu University, Gifu, Japan

Keywords: Chlamydiales, Chlamydia pecorum, Chlamydia-related bacteria, Japan, pet bird

JOURNAL OPEN ACCESS
Supplementary material

2023 Volume 85 Issue 9 Pages 907-911

DOI https://doi.org/10.1292/jvms.23-0185

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Abstract

Chlamydia-related bacteria of the Chlamydiales order have recently been described as emerging pathogens that cause pneumonia and abortion in animals and humans. We investigated the presence of Chlamydiales using real-time polymerase chain reaction (PCR) by targeting the 16S rRNA gene of a broad range of Chlamydiales in 827 fecal samples from pet birds kept in individual homes in Japan. Of the 827 samples, 493 (59.6%) tested positive for the Chlamydiales 16S rRNA gene in the real-time PCR assay. We determined the nucleic acid sequences of PCR products from 17 Chlamydiales strains. A homology search and phylogenetic analysis using these sequences confirmed that the detected Chlamydiales included C. pecorum and a broad range of Chlamydia-related bacteria. To the best of our knowledge, this is the first study to detect a wide range of Chlamydia-related bacteria in birds.

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