Genetic differences between C57BL/6 substrains affect the process of testis          differentiation in YPOS mice

Toshifumi YOKOYAMA; Yuuka MIURA; Anzu YAMAMOTO; Chinatsu HASEGAWA; Kohei KAWANISHI; Nanako TAKADA; Takuya OMOTEHARA; Tetsushi HIRANO; Yohei MANTANI; Takanori MIKI; Nobuhiko HOSHI

doi:10.1292/jvms.18-0621

Anatomy

Genetic differences between C57BL/6 substrains affect the process of testis differentiation in Y^POS mice

Toshifumi YOKOYAMA, Yuuka MIURA, Anzu YAMAMOTO, Chinatsu HASEGAWA, Kohei KAWANISHI, Nanako TAKADA, Takuya OMOTEHARA, Tetsushi HIRANO, Yohei MANTANI, Takanori MIKI, Nobuhiko HOSHI

Author information

Toshifumi YOKOYAMA
Department of Animal Science, Graduate School of Agricultural Science, Kobe University, 1-1 Rokkodai, Nada-ku, Kobe, Hyogo 657-8501, Japan
Yuuka MIURA
Department of Animal Science, Graduate School of Agricultural Science, Kobe University, 1-1 Rokkodai, Nada-ku, Kobe, Hyogo 657-8501, Japan
Anzu YAMAMOTO
Department of Animal Science, Graduate School of Agricultural Science, Kobe University, 1-1 Rokkodai, Nada-ku, Kobe, Hyogo 657-8501, Japan
Chinatsu HASEGAWA
Department of Animal Science, Graduate School of Agricultural Science, Kobe University, 1-1 Rokkodai, Nada-ku, Kobe, Hyogo 657-8501, Japan
Kohei KAWANISHI
Department of Animal Science, Graduate School of Agricultural Science, Kobe University, 1-1 Rokkodai, Nada-ku, Kobe, Hyogo 657-8501, Japan
Nanako TAKADA
Department of Animal Science, Graduate School of Agricultural Science, Kobe University, 1-1 Rokkodai, Nada-ku, Kobe, Hyogo 657-8501, Japan
Takuya OMOTEHARA
Department of Anatomy, Tokyo Medical University, 6-1-1 Shinjuku, Shinjuku-ku, Tokyo 160-8402, Japan
Tetsushi HIRANO
Division of Drug and Structural Research, Life Science Research Center, University of Toyama, 2630 Sugitani, Toyama 930-0194, Japan
Yohei MANTANI
Department of Animal Science, Graduate School of Agricultural Science, Kobe University, 1-1 Rokkodai, Nada-ku, Kobe, Hyogo 657-8501, Japan
Takanori MIKI
Departments of Anatomy and Neurobiology, Faculty of Medicine, Kagawa University, 1750-1 Ikenobe, Miki-cho, Kagawa 761-0793, Japan
Nobuhiko HOSHI
Department of Animal Science, Graduate School of Agricultural Science, Kobe University, 1-1 Rokkodai, Nada-ku, Kobe, Hyogo 657-8501, Japan

Keywords: C57BL/6, Mus musculus poschiavinus, sex reversal, substrain, testis differentiation

JOURNAL FREE ACCESS

2019 Volume 81 Issue 4 Pages 608-611

DOI https://doi.org/10.1292/jvms.18-0621

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Abstract

C57BL/6J-XY^POS(B6J-XY^POS) mice, which have the Y chromosome derived from Mus musculus poschiavinus on a B6J genetic background, form ovotestes or ovaries. Previously, we replaced the genetic background of B6J-XY^POS mice with B6N and found that individuals with testes also appeared in addition to those with ovaries or ovotestes. To investigate the effect of the B6J genetic sequence on the testis differentiation, the genetic background of B6N-XY^POS mice was replaced with B6J again. The recovery of the B6J genetic background significantly decreased the incidence of testes; only ovaries developed. These results indicate that the testicular differentiation process tends to be perturbed especially in the B6J substrain. This shows the importance of substrain differences in mice usually treated as B6 collectively.

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