Emergence of Salmonella enterica Serovar Infantis Harboring IncI1 Plasmid with blaCTX-M-14 in a Broiler Farm in Japan

Mitsuhiro KAMEYAMA; Takehisa CHUMA; Tomoki YOKOI; Junko YABATA; Kiyoshi TOMINAGA; Daisuke MIYASAKO; Hiroyuki IWATA; Karoku OKAMOTO

doi:10.1292/jvms.11-0488

Public Health

Emergence of Salmonella enterica Serovar Infantis Harboring IncI1 Plasmid with bla_CTX-M-14 in a Broiler Farm in Japan

Mitsuhiro KAMEYAMA, Takehisa CHUMA, Tomoki YOKOI, Junko YABATA, Kiyoshi TOMINAGA, Daisuke MIYASAKO, Hiroyuki IWATA, Karoku OKAMOTO

Author information

Mitsuhiro KAMEYAMA
Yamaguchi Prefectural Institute of Public Health and Environment, 2–5–67 Aoi, Yamaguchi 753–0821, Japan
Takehisa CHUMA
Laboratory of Veterinary Public Health, Joint Faculty of Veterinary Medicine, Kagoshima University, 1–21–24 Korimoto, Kagoshima 890–0065, Japan
Tomoki YOKOI
Laboratory of Veterinary Public Health, Joint Faculty of Veterinary Medicine, Kagoshima University, 1–21–24 Korimoto, Kagoshima 890–0065, Japan
Junko YABATA
Yamaguchi Prefectural Institute of Public Health and Environment, 2–5–67 Aoi, Yamaguchi 753–0821, Japan
Kiyoshi TOMINAGA
Yamaguchi Prefectural Institute of Public Health and Environment, 2–5–67 Aoi, Yamaguchi 753–0821, Japan
Daisuke MIYASAKO
Laboratory of Veterinary Public Health, Joint Faculty of Veterinary Medicine, Kagoshima University, 1–21–24 Korimoto, Kagoshima 890–0065, Japan
Hiroyuki IWATA
Department of Veterinary Medicine, Joint Faculty of Veterinary Medicine, Yamaguchi University, 1677–1 Yoshida, Yamaguchi 753–8515, Japan
Karoku OKAMOTO
Laboratory of Veterinary Public Health, Joint Faculty of Veterinary Medicine, Kagoshima University, 1–21–24 Korimoto, Kagoshima 890–0065, Japan

Keywords: β-lactamase, broiler, CTX-M-14, extended-spectrum plasmid replicon typing, Salmonella Infantis

JOURNAL FREE ACCESS

2012 Volume 74 Issue 9 Pages 1213-1216

DOI https://doi.org/10.1292/jvms.11-0488

Browse “Advance online publication” version

Details

Abstract

Cefotaxime (CTX)-resistant and -susceptible Salmonella enterica serovar Infantis isolates obtained from broilers raised on a farm in January 2010 in Japan were characterized to establish their resistance determinants. The CTX-resistant isolates produced CTX-M-14 extended-spectrum β-lactamase and harbored 2 distinct plasmid of approximately 140- and 95-kb, whereas the CTX-susceptible isolates harbored one 140-kb plasmid. The 95-kb plasmids were replicon typed as IncI1 carrying the bla_CTX-M-14 gene, while the 140-kb plasmids were IncP and harbored the aphA1, aadA1, tetA, and sul1 genes. Genetic fingerprinting by pulsed-field gel electrophoresis revealed similar macrorestriction profiles amongst CTX-resistant and susceptible isolates, suggesting a clonal relationship. The presence of CTX-resistant S. Infantis on a broiler farm has occurred through the acquisition of IncI1 resistance plasmid.

Corresponding author

Register with J-STAGE for free!