Expressions of Lipid Oxidation Markers, Nε-Hexanoyl Lysine and Acrolein in Cisplatin-Induced Nephrotoxicity in Rats

Akihiko SUGIYAMA; Jing SUN; Masaaki NISHINOHARA; Yuka FUJITA; Aino MASUDA; Tairin OCHI; Takashi TAKEUCHI

doi:10.1292/jvms.10-0476

Toxicology

Expressions of Lipid Oxidation Markers, N^ε-Hexanoyl Lysine and Acrolein in Cisplatin-Induced Nephrotoxicity in Rats

Akihiko SUGIYAMA, Jing SUN, Masaaki NISHINOHARA, Yuka FUJITA, Aino MASUDA, Tairin OCHI, Takashi TAKEUCHI

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Keywords: acrolein, cisplatin, N^ε-hexanoyl lysine, nephrotoxicity, oxidative damage

JOURNAL FREE ACCESS

2011 Volume 73 Issue 6 Pages 821-826

DOI https://doi.org/10.1292/jvms.10-0476

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Abstract

The purpose of this study was to evaluate whether N^ε-hexanoyl lysine (N^ε-HEL) and acrolein reflect the severity of cisplatin-induced nephrotoxicity. Immunoexpression of N^ε-HEL and acrolein in kidneys and their urinary concentration were examined up to day 4 post-cisplatin injection in rats. Cisplatin-induced tubular injury was observed histopathologically on days 2-4 after injection and became more severe time-dependently. On days 2-4, N^ε-HEL and acrolein were immunostained in the cytoplasm of damaged tubular cells. Their immunostaining intensity and urinary levels increased as tubular injury became more severe. These results suggest that expressions of N^ε-HEL and acrolein were associated with the pathogenesis of cisplatin-induced nephrotoxicity.

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