Assessment of Oxidative Stress in the Spontaneously Hypertensive Rat Brain Using Electron Spin Resonance (ESR) Imaging and in Vivo L-Band ESR

Masaichi-Chang-il LEE; Hirofumi SHOJI; Hiroyuki MIYAZAKI; Fumihiko YOSHINO; Norio HORI; Minoru TOYODA; Yukio IKEDA; Kazunori ANZAI; Nobuo IKOTA; Toshihiko OZAWA

doi:10.1291/hypres.27.485

Experimental studies

Assessment of Oxidative Stress in the Spontaneously Hypertensive Rat Brain Using Electron Spin Resonance (ESR) Imaging and in Vivo L-Band ESR

Masaichi-Chang-il LEE, Hirofumi SHOJI, Hiroyuki MIYAZAKI, Fumihiko YOSHINO, Norio HORI, Minoru TOYODA, Yukio IKEDA, Kazunori ANZAI, Nobuo IKOTA, Toshihiko OZAWA

Author information

Masaichi-Chang-il LEE
Department of Clinical Care Medicine, Division of Pharmacology and ESR Laboratories, Kanagawa Dental College
Hirofumi SHOJI
Department of Clinical Care Medicine, Division of Pharmacology and ESR Laboratories, Kanagawa Dental College
Hiroyuki MIYAZAKI
Department of Clinical Care Medicine, Division of Pharmacology and ESR Laboratories, Kanagawa Dental College
Fumihiko YOSHINO
Department of Clinical Care Medicine, Division of Pharmacology and ESR Laboratories, Kanagawa Dental College
Norio HORI
Department of Oral and Maxillofacial Rehabilitation, Division of Prosthetics, Kanagawa Dental College
Minoru TOYODA
Department of Oral and Maxillofacial Rehabilitation, Division of Prosthetics, Kanagawa Dental College
Yukio IKEDA
Department of Neurosurgery, Tokyo Medical University, Hachioji Medical Center
Kazunori ANZAI
National Institute of Radiological Sciences
Nobuo IKOTA
National Institute of Radiological Sciences
Toshihiko OZAWA
National Institute of Radiological Sciences

Keywords: electron spin resonance imaging, L-band electron spin resonance, brain, oxidative stress, spontaneously hypertensive rat

JOURNAL FREE ACCESS

2004 Volume 27 Issue 7 Pages 485-492

DOI https://doi.org/10.1291/hypres.27.485

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Abstract

This study examined the blood brain barrier (BBB)-permeable nitroxyl compound, 3-methoxycarbonyl-2,2,5,5-tetramethylpyrrolidine-1-oxyl (MC-PROXYL), as a spin probe for the assessment of oxidative stress in the brain by electron spin resonance (ESR) imaging and in vivo L-band ESR. Preliminary comparisons were made by ESR imaging of MC-PROXYL in the isolated brains of normal Wistar-Kyoto rats (WKY), spontaneously hypertensive rats (SHR), and stroke prone SHR (SHRSP). The decay of the ESR images of MC-PROXYL in the isolated brains was faster in SHR than in normal WKY, but was only moderate in SHRSP. In addition, the decay rate of MC-PROXYL in the heads of live rats, as measured noninvasively by L-band ESR, was faster in SHR than in WKY, and was slower in SHR than in SHRSP. Taken together, our data suggest that the oxidative stress of SHR is not as high as that in high oxidative stress animal models such as SHRSP. This is the first study to present reconstructed 3D images of the distribution of MC-PROXYL in the isolated SHR brain. The ESR technique employed herein appears to be a powerful tool for evaluating oxidative stress and for detecting the region of oxidative stress in the brain of SHR. (Hypertens Res 2004; 27: 485-492)

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