A NEW CELL LINE FROM LARVAL FAT BODIES OF THE BOLLWORM, HELICOVERPA ARMIGERA (LEPIDOPTERA: NOCTUIDAE)

HUAN ZHANG; YONG-AN ZHANG; QILIAN QIN; YUZHU WANG; XUAN LI; LIN MIAO; ZHENXIAN YIN; AIJUN ZHANG; LIANGJIAN QU; CUI DING

doi:10.1290/0605033.1

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1 November 2006 A NEW CELL LINE FROM LARVAL FAT BODIES OF THE BOLLWORM, HELICOVERPA ARMIGERA (LEPIDOPTERA: NOCTUIDAE)

HUAN ZHANG, YONG-AN ZHANG, QILIAN QIN, YUZHU WANG, XUAN LI, LIN MIAO, ZHENXIAN YIN, AIJUN ZHANG, LIANGJIAN QU, CUI DING

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In Vitro Cellular & Developmental Biology - Animal, 42(10):290-293 (2006). https://doi.org/10.1290/0605033.1

Abstract

A new cell line, designated IOZCAS-Ha-I, was initiated from the fat body of larvae of Helicoverpa armigera (Lepidoptera: Noctuidae) in TNM-FH medium containing 10% fetal bovine serum. Spherical cells were predominant among the various cell types. The cell line showed a typical lepidopteran chromosome pattern ranging from 58 to 239 chromosomes in the majority of the cells. It was confirmed to have originated from the H. armigera by the DNA amplification– fingerprinting polymerase chain reaction (DAF-PCR) technique. The new cell line was only slightly susceptible to the multiple nucleocapsid nuclear polyhedrosis viruses (NPV) from H. armigera.

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HUAN ZHANG, YONG-AN ZHANG, QILIAN QIN, YUZHU WANG, XUAN LI, LIN MIAO, ZHENXIAN YIN, AIJUN ZHANG, LIANGJIAN QU, and CUI DING "A NEW CELL LINE FROM LARVAL FAT BODIES OF THE BOLLWORM, HELICOVERPA ARMIGERA (LEPIDOPTERA: NOCTUIDAE)," In Vitro Cellular & Developmental Biology - Animal 42(10), 290-293, (1 November 2006). https://doi.org/10.1290/0605033.1

Received: 11 July 2006; Accepted: 1 August 2006; Published: 1 November 2006

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