As the Ne1 locus had already been located on the long arm of chromosome 5B (Zeven 1972), Chinese Spring ditelosomic for 5BL (ne 1/ne 1 ne 2/ne 2) was crossed with Prelude (Ne 1/Ne 1 ne 2/ne 2) and the resulting F₁'s monotelodisomic for 5BL and heterozygous for the Ne 1 locus were testcrossed to Atlas 66 (ne 1/ne I Ne 2/Ne 2). The Ne1-centro-mere distance obtained from combined data of necrosis phenotypes and chrolrfosome comple-ments was 10.5±2.0 map units. From the results of crosses including the necrosis testers, Prelude and Atlas 66, the line monotelosomic for 2BL-ditelosomic for 2BS of Chinese Spr-ing was confirmed to carry Ne 2 on telosome 2BS. Then, F₁'s monotelodisomic for 2BS and heterozygous for the Ne 2 Iocus which were derived from the cross, monotelo 2BL-ditelo 2BS×disomic Chinese Spring were test-crossed with Triticum durum LD 222 (Ne 1/ Ne 1 ne 2/ne 2). The Ne 2-centromere dis-tance obtained was 9.4±1.5 map units.