The purpose of this study was to elucidate what gap junctional protein connexins (Cxs) were expressed in human nasal mucosa and polyps, and to analyze the role of gap junctions in intercellular calcium waves elicited by mechanical stimulation in cultured human nasal epithelial cells. Immunoconfocal labeling in human nasal tissue showed that Cx26 was expressed between epithelial cells, whereas Cx43 existed in stromal cells. No specific immunolabeling for Cx32 was observed. The capacity of gap junctional intercellular communication in cultured nasal epithelial cells was tested by microinjection with Lucifer yellow, which was transferred into neighboring epithelial cells. To analyze intercellular propagation of calcium waves, cultured nasal epithelial cells were loaded with fluo−3/AM, and then treated with heptanol (a gap junction inhibitor) or suramin (a P₂−purinergic receptor inhibitor). Both heptanol and suramin reduced intercellular propagation of the calcium wave to 65% and 66%, respectively. Our study indicates that chronic inflammation doesn’t significantly influence the pattern of connexin expression in human nasal mucosa, and suggests that calcium waves elicited by mechanical stimulation in cultured human nasal epithelium propagate between epithelial cells through at least two pathways, i.e, gap junctions and P₂−purinergic receptors.