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 | Construction of a BAC library for Haplochromis chilotes, a cichlid fish from Lake Victoria
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| | Masakatsu Watanabe1), Naoki Kobayashi1), Asao Fujiyama2)3) and Norihiro Okada1) |
| 1) Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology
2) Information Research Division, National Institute of Informatics
3) RIKEN Genomic Sciences Center |
| | Cichlid fishes in Lake Victoria are model organisms for studying rapid radiation and speciation. On the way to examine the molecular basis of how these cichlid fishes achieved such a remarkable morphological diversification, we constructed a bacterial artificial chromosome (BAC) library derived from a cichlid species, Haplochromis chilotes, from Lake Victoria. The library includes 157,056 clones with the average insert size of 128 kb, corresponding to a 10-fold coverage of the H. chilotes genome. Given that the cichlid fishes endemic to Lake Victoria are closely related to one another phylogenetically and their genomes are nearly identical, this BAC library can be utilized to isolate genes from the more than 200 Haplochromine cichlid species in Lake Victoria.
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| | cichlid BAC library Lake Victoria |
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| To cite this article: | | Masakatsu Watanabe, Naoki Kobayashi, Asao Fujiyama and Norihiro Okada. “Construction of a BAC library for Haplochromis chilotes, a cichlid fish from Lake Victoria” Genes Genet. Syst. Vol. 78 103-105 (2003) . | |
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| doi:10.1266/ggs.78.103 | | JOI JST.JSTAGE/ggs/78.103 |
| Copyright (c) 2003 by The Genetics Society of Japan |
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