Improving enzyme immobilization in mesocellular siliceous foams by microwave irradiation
Section snippets
Materials
Penicillin acylase (EC 3.5.1.11, 796 U/ml, 10 mg/ml) from Bacillus megaterium, was obtained from Zhejiang Shunfenghaidier (Dongyang, P.R. China). Penicillin G potassium was from Hua Xing Medicines Factory (Xinxiang, P.R. China). Papain (EC 3.4.22.2, 1196.4 U/mg) was purchased from Guangxi Pangbo Biotechnology (Nanning, P.R. China). Poly (ethylene glycol)-poly-block (propyleneglycol)-poly-block (ethylene glycol) (P123) was from Sigma-Aldrich of the highest grade commercially available. Other
Characterization of the support
The nitrogen adsorption-desorption isotherms and pore size distribution are shown in Fig. 1. The data indicated that uniformly sized and large spherical cells were interconnected by uniform windows and could form a continuous 3-D pore system. This result showed that the structure of MCFs was retained during microwave digestion of the organic templates.
Effect of microwave power output level on the activity of the immobilized enzyme
In organic syntheses, microwave irradiation can accelerate organic transformation and reduce reaction times from days and hours to minutes and
Acknowledgments
This work was supported by The National High Technology Research and Development Program of China (863 Program, no. 2006AA02Z211), The National Natural Science Foundation of China (20376034), The Natural Science Foundation of Jiangsu Province of China (BK2006181), and The Foundation of Jiangsu Province of China for College Postgraduate Students in Innovation Engineering (2007). We thank Shayne X. Wang, Ph.D. for revising the manuscript.
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