Optimization of gellan gum production by Sphingomonas paucimobilis ATCC 31461 with nonionic surfactants using central composite design
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Media and culture conditions
Gellan production was carried out using Sphingomonas paucimobilis ATCC 31461. The strain was maintained in slant culture on YPG medium (2% glucose, 0.3% yeast extract, 0.5% peptone). Gellan fermentation was carried out in a medium with the following composition (per l): sucrose, 40 g; monosodium glutamate, 1.25 g; casaminoacid, 1 g; KH2PO4., 3 g; Na2HPO4, 5 g; K2SO4, 1 g; CaCl2·2H2O, 3 mmol; MnSO4·7H2O, 1.5 mmol; NaCl, 1 g; pH 6.5. Inoculum was developed by transferring one loopfull of the
Effect of nonionic surfactants on biomass, gellan production and rheology
The maximum biomass and gellan productions obtained from the shake flask fermentation were 4.16 g/l and 8.63 g/l, respectively. The biomass and gellan productions obtained from the shake flask fermentation with Triton X-100, added at different concentrations are given in Fig. 1. Biomass increased with increasing surfactant concentration up to 1 g/l and showed no change above this level. Fermentation with Triton X-100 resulted in higher cell biomass at 5.06 g/l and those with Tween 80 and Tween
References (26)
- et al.
Structure of the acidic extracellular gelling polysaccharide produced by Pseudomonas elodea
Carbohydr. Res.
(1983) - et al.
Effect of Tween 80 and oleic acid on ligninases production by Phanerochaete chrysosporium INA-12
Enzyme Microb. Technol.
(1987) - et al.
Detergents improve yield and polymer quality in cultures of Xanthomonas campestris
Enzyme Microb. Technol.
(1996) - et al.
Specific colour reaction of methyl pentoses and a spectrophotometric micromethod for their determination
J. Biol. Chem.
(1948) - et al.
A Modified uronic acid carbazole reaction
Anal. Biochem.
(1962) - et al.
Aeration
Ann. Rep. Ferment. Proc.
(1983) Biotechnically produced carbohydrate with functional properties for use in food system
Food Biotechnol.
(1990)- et al.
Rheological evaluation of gelrite in situ for ophthalmic use
Eur. J. Pharm. Sci.
(1998) - et al.
Physical characteristics and release behaviour of salbutamol sulphate beads prepared with different ionic polysaccharide
Drug Dev. Ind. Pharm.
(1996) Isolation of mutant strain of Pseudomonas sp. ATCC 31461 exhibiting elevated polysaccharide production
J. Ind. Microbiol. Biotechnol.
(2002)
Effect of environmental factors and carbohydrate on gellan gum production in batch mode
Appl. Biochem. Biotechnol.
Fermentative production of gellan using Sphingomonas paucimobilis
Process Biochem.
Improvement in production and quality of gellan gum under high dissolved oxygen tension levels
Biotechnol. Lett.
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