A simple and sensitive method was developed for the determination of total iodine in iodine-enriched eggs (IEs). The egg yolk and white of IE were homogenized with ethanol. The homogenate was decomposed by oxygen flask combustion with an alkaline absorbing solution. After the combustion, iodide (I^-) obtained in the absorbing solution was concentrated by solid-phase extraction with an anion-exchange column. The concentrated I^- was determined by reversed-phase HPLC with a UV detector. The reproducibility obtained for the determination was good. The recoveries of iodine added to the egg yolk and white were about 90%. The method was applied to the determination of total iodine in commercial IEs.